The HSP terminator of Arabidopsis thaliana induces a high level of miraculin accumulation in transgenic tomatoes

Tadayoshi Hirai, Natsuko Kurokawa, Narendra Duhita, Kyoko Hiwasa-Tanase, Kazuhisa Kato, Ko Kato, Hiroshi Ezura

Research output: Contribution to journalArticlepeer-review

20 Citations (Scopus)

Abstract

High-level accumulation of the target recombinant protein is a significant issue in heterologous protein expression using transgenic plants. Miraculin, a taste-modifying protein, was accumulated in transgenic tomatoes using an expression cassette in which the miraculin gene was expressed by the cauliflower mosaic virus (CaMV) 35S promoter and the heat shock protein (HSP) terminator (MIR-HSP). The HSP terminator was derived from heat shock protein 18.2 in Arabidopsis thaliana. Using this HSP-containing cassette, the miraculin concentration in T 0 transgenic tomato lines was 1.4-13.9% of the total soluble protein (TSP), and that in the T 1 transgenic tomato line homozygous for the miraculin gene reached 17.1% of the TSP. The accumulation level of the target protein was comparable to levels observed with chloroplast transformation. The high-level accumulation of miraculin in T 0 transgenic tomato lines achieved by the HSP terminator was maintained in the successive T 1 generation, demonstrating the genetic stability of this accumulation system.

Original languageEnglish
Pages (from-to)9942-9949
Number of pages8
JournalJournal of Agricultural and Food Chemistry
Volume59
Issue number18
DOIs
Publication statusPublished - 2011 Sep 28
Externally publishedYes

Keywords

  • CaMV 35S promoter
  • HSP terminator
  • high-level expression
  • miraculin
  • transgenic tomato plants

ASJC Scopus subject areas

  • Chemistry(all)
  • Agricultural and Biological Sciences(all)

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