The gravity-induced re-localization of auxin efflux carrier CsPIN1 in cucumber seedlings: Spaceflight experiments for immunohistochemical microscopy

Chiaki Yamazaki, Nobuharu Fujii, Yutaka Miyazawa, Motoshi Kamada, Haruo Kasahara, Ikuko Osada, Toru Shimazu, Yasuo Fusejima, Akira Higashibata, Takashi Yamazaki, Noriaki Ishioka, Hideyuki Takahashi

    Research output: Contribution to journalArticlepeer-review

    14 Citations (Scopus)

    Abstract

    Reorientation of cucumber seedlings induces re-localization of CsPIN1 auxin efflux carriers in endodermal cells of the transition zone between hypocotyl and roots. This study examined whether the re-localization of CsPIN1 was due to the graviresponse. Immunohistochemical analysis indicated that, when cucumber seedlings were grown entirely under microgravity conditions in space, CsPIN1 in endodermal cells was mainly localized to the cell side parallel to the minor axis of the elliptic cross-section of the transition zone. However, when cucumber seeds were germinated in microgravity for 24 h and then exposed to 1g centrifugation in a direction crosswise to the seedling axis for 2 h in space, CsPIN1 was re-localized to the bottom of endodermal cells of the transition zone. These results reveal that the localization of CsPIN1 in endodermal cells changes in response to gravity. Furthermore, our results suggest that the endodermal cell layer becomes a canal by which auxin is laterally transported from the upper to the lower flank in response to gravity. The graviresponse-regulated re-localization of CsPIN1 could be responsible for the decrease in auxin level, and thus for the suppression of peg formation, on the upper side of the transition zone in horizontally placed seedlings of cucumber.

    Original languageEnglish
    Article number16030
    Journalnpj Microgravity
    Volume2
    DOIs
    Publication statusPublished - 2016 Jan 7

    ASJC Scopus subject areas

    • Medicine (miscellaneous)
    • Materials Science (miscellaneous)
    • Biochemistry, Genetics and Molecular Biology (miscellaneous)
    • Agricultural and Biological Sciences (miscellaneous)
    • Physics and Astronomy (miscellaneous)
    • Space and Planetary Science

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