The GLABRA2 homeodomain protein directly regulates CESA5 and XTH17 gene expression in Arabidopsis roots

Rumi Tominaga-Wada, Mineko Iwata, Junji Sugiyama, Toshihisa Kotake, Tetsuya Ishida, Ryusuke Yokoyama, Kazuhiko Nishitani, Kiyotaka Okada, Takuji Wada

Research output: Contribution to journalArticlepeer-review

44 Citations (Scopus)

Abstract

Arabidopsis root hair formation is determined by the patterning genes CAPRICE (CPC), GLABRA3 (GL3), WEREWOLF (WER) and GLABRA2 (GL2), but little is known about the later changes in cell wall material during root hair formation. A combined Fourier-transform infrared microspectroscopy-principal components analysis (FTIR-PCA) method was used to detect subtle differences in the cell wall material between wild-type and root hair mutants in Arabidopsis. Among several root hair mutants, only the gl2 mutation affected root cell wall polysaccharides. Five of the 10 genes encoding cellulose synthase (CESA1-10) and 4 of 33 xyloglucan endotransglucosylase (XTH1-33) genes in Arabidopsis are expressed in the root, but only CESA5 and XTH17 were affected by the gl2 mutation. The L1-box sequence located in the promoter region of these genes was recognized by the GL2 protein. These results indicate that GL2 directly regulates cell wall-related gene expression during root development.

Original languageEnglish
Pages (from-to)564-574
Number of pages11
JournalPlant Journal
Volume60
Issue number3
DOIs
Publication statusPublished - 2009 Nov

Keywords

  • CESA5
  • Cell wall
  • Fourier transform infrared spectroscopy
  • GLABRA2
  • Root
  • XTH17

ASJC Scopus subject areas

  • Genetics
  • Plant Science
  • Cell Biology

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