The gene for interleukin-21 receptor is the partner of BCL6 in t(3;16)(q27;p11), which is recurrently observed in diffuse large B-cell lymphoma

Chiyoko Ueda, Takashi Akasaka, Masayuki Kurata, Yoshitomo Maesako, Momoko Nishikori, Ryo Ichinohasama, Kazunori Imada, Takashi Uchiyama, Hitoshi Ohno

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34 Citations (Scopus)

Abstract

BCL6 translocation affecting the chromosomal band 3q27 can involve a number of non-immunoglobulin (non-IG) gene loci as partners. We report here that the gene for interleukin-21 receptor (IL-21R) is the partner of BCL6 in t(3;16)(q27;p11) translocation. The two break-points on 16p11 of a lymphoma cell line YM and case no. 1012 with diffuse large B-cell lymphoma, both of which carried t(3;16), were localized within the ∼27-kb intron 1 of IL-21R. As a result of t(3;16), the promoter region of IL-21R was substituted for the regulatory sequences of BCL6 in the same transcriptional orientation. Reverse transcriptase-mediated polymerase chain reaction revealed chimeric mRNA consisting of two non-coding exons 1a/1b of IL-21R and coding exons of BCL6 in both lymphoma cells. Fluorescence in situ chromosomal hybridization of YM metaphase cells revealed fusion signals that contained both the BCL6 and IL-21R sequences on the der(3)t(3;16) chromosome. IL-21R was actively transcribed in YM cells, while BCL6 that was under the control of the IL-21R promoter was only moderately expressed at the mRNA and protein level. We constructed expression plasmid of BCL6 that followed the promoter sequences of IL-21R. COS-7 cells transiently transfected with the plasmid expressed high level Bcl-6 protein and displayed nuclear staining with a characteristic punctate pattern by immunofluorescence, indicating that expression of BCL6 can be enhanced by t(3;16). This study added to the list of non-IG partners of BCL6 translocations a new class of gene, i.e. cytokine receptor gene, the expression of which is closely associated with lymphoid cells.

Original languageEnglish
Pages (from-to)368-376
Number of pages9
JournalOncogene
Volume21
Issue number3
DOIs
Publication statusPublished - 2002 Jan 17
Externally publishedYes

Keywords

  • BCL6 gene
  • Diffuse large B-cell lymphoma
  • Interleukin-21 receptor gene
  • Non-immunoglobulin gene
  • t(3;16)(q27;p11) translocation

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cancer Research

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