The gene for component-II of botulinum C2 toxin

Koichi Kimura, Toru Kubota, Iwao Ohishi, Emiko Isogai, Hiroshi Isogai, Nobuhiro Fujii

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

The gene encoding component-II of the Clostridium botulinum C2 toxin was cloned from the chromosomal DNA of C. botulinum type C strain (C)-203U28, and the nucleotide sequence was determined. The gene (bc2h) encodes a protein with 721 amino acid residues and is located at 247 bp downstream of the gene for component-I. The N-terminal 16 amino acids were identical to those obtained by analysis of the purified component-II toxin. The ORF for bc2h had only 39% homology at the amino acid level with the C. perfringens iota-lb protein and an ATP/GTP binding site which is present in the iota-lb protein is missing from the protein encoded by bc2h. Both genes had a homologous region that predicts a transmembrane segment.

Original languageEnglish
Pages (from-to)27-34
Number of pages8
JournalVeterinary Microbiology
Volume62
Issue number1
DOIs
Publication statusPublished - 1998 Apr 30

Keywords

  • Clostridium botulinum
  • Component-II
  • Gene cloning
  • Toxins

ASJC Scopus subject areas

  • Microbiology
  • veterinary(all)

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  • Cite this

    Kimura, K., Kubota, T., Ohishi, I., Isogai, E., Isogai, H., & Fujii, N. (1998). The gene for component-II of botulinum C2 toxin. Veterinary Microbiology, 62(1), 27-34. https://doi.org/10.1016/S0378-1135(98)00195-3