The gene expression landscape of thermogenic skunk cabbage suggests critical roles for mitochondrial and vacuolar metabolic pathways in the regulation of thermogenesis

Yasuko Ito-Inaba, Yamato Hida, Hideo Matsumura, Hiromi Masuko, Fumiko Yazu, Ryohei Terauchi, Masao Watanabe, Takehito Inaba

    Research output: Contribution to journalArticle

    11 Citations (Scopus)

    Abstract

    Floral thermogenesis has been described in several plant species. Because of the lack of comprehensive gene expression profiles in thermogenic plants, the molecular mechanisms by which floral thermogenesis is regulated remain to be established. We examined the gene expression landscape of skunk cabbage (Symplocarpus renifolius) during thermogenic and post-thermogenic stages and identified expressed sequence tags from different developmental stages of the inflorescences using super serial analysis of gene expression (SuperSAGE). In-depth analysis suggested that cellular respiration and mitochondrial functions are significantly enhanced during the thermogenic stage. In contrast, genes involved in stress responses and protein degradation were significantly up-regulated during post-thermogenic stages. Quantitative comparisons indicated that the expression levels of genes involved in cellular respiration were higher in thermogenic spadices than in Arabidopsis inflorescences. Thermogenesis-associated genes seemed to be expressed abundantly in the peripheral tissues of the spadix. Our results suggest that cellular respiration and mitochondrial metabolism play key roles in heat production during floral thermogenesis. On the other hand, vacuolar cysteine protease and other degradative enzymes seem to accelerate senescence and terminate thermogenesis in the post-thermogenic stage. Floral thermogenesis has been described in several plant taxa including gymnosperms as well as eudicots and monocots. Because of the lack of comprehensive gene expression profiles in thermogenic plants, the molecular mechanisms by which floral thermogenesis is regulated remain to be established. We examined the gene expression landscape of skunk cabbage (Symplocarpus renifolius) during thermogenic and post-thermogenic stages. Our findings suggest that cellular respiration and mitochondrial metabolism play key roles in heat production during floral thermogenesis, while vacuolar cysteine protease and other degradative enzymes accelerate senescence and terminate thermogenesis in the post-thermogenic stage.

    Original languageEnglish
    Pages (from-to)554-566
    Number of pages13
    JournalPlant, Cell and Environment
    Volume35
    Issue number3
    DOIs
    Publication statusPublished - 2012 Mar 1

    Keywords

    • Floral thermogenesis
    • Low temperature
    • Plant mitochondria
    • Respiration
    • SuperSAGE

    ASJC Scopus subject areas

    • Physiology
    • Plant Science

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