The gelsolin/fragmin family protein identified in the higher plant Mimosa pudica

Sawako Yamashiro, Kazuhisa Kameyama, Nobuyuki Kanzawa, Tbru Tamiya, Issei Mabuchi, Takahide Tsuchiya

    Research output: Contribution to journalArticlepeer-review

    31 Citations (Scopus)

    Abstract

    Mimosa pudica L. rapidly closes its leaves and bends its petioles downward when mechanically stimulated. It has been suggested that the actin cytoskeleton is involved in the bending motion since both cytochalasin B and phalloidin inhibit the motion. In order to clarify the mechanism by which the actin cytoskeleton functions in the motion, we attempted to find actin-modulating proteins in the M. pudica plant by DNase I-affinity column chromatography. The EGTA-eluate from the DNase I column contained proteins with apparent molecular masses of 90- and 42-kDa. The 42-kDa band consisted of two closely migrating components: the slower migrating component was actin while the faster migrating components was a distinct protein. The eluate showed an activity to sever actin filaments and to enhance the rate of polymerization of actin, both in a Ca2+-dependent manner. Microsequencing of the faster migrating 42-kDa protein revealed its similarity to proteins in the gelsolin/fragmin family. Our results provide the first biochemical evidence for the presence in a higher plant of a gelsolin/fragmin family actin-modulating protein that severs actin filament in a Ca2+-dependent manner.

    Original languageEnglish
    Pages (from-to)243-249
    Number of pages7
    JournalJournal of biochemistry
    Volume130
    Issue number2
    DOIs
    Publication statusPublished - 2001 Aug

    Keywords

    • Actin
    • Actin-modulating protein
    • F-actin severing protein
    • Gelsolin/fragmin
    • Mimosa pudica

    ASJC Scopus subject areas

    • Biochemistry
    • Molecular Biology

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