The fungal hydrophobin RolA promotes PBSA degradation

Toru Takahashi, Hiroshi Maeda, Yohei Yamagata, Katsuya Gomi, Keietsu Abe

    Research output: Contribution to conferencePaperpeer-review

    Abstract

    When an industrial fungus Aspergillus oryzae was cultivated in liquid medium containing the biodegradable polyester polybutylene succinate-coadipate (PBSA), the rolA gene encoding hydrophobin RolA was highly transcribed. Preincubation of PBSA with RolA stimulated PBSA degradation by CutL1, suggesting that RolA bound to a PBSA surface was required for the stimulation. Immunostaining revealed that PBSA films coated with RolA specifically adsorbed CutL1. QCM analyses further demonstrated that RolA attached to a hydrophobic sensor chip specifically adsorbed CutL1. These results suggest that RolA adsorbed to the hydrophobic surface of PBSA recruits CutL1, resulting in condensation of CutL1 on the PBSA surface and consequent stimulation of PBSA hydrolysis. A fluorescence recovery after photobleaching (FRAP) experiment on PBSA films coated with FITC-labeled RolA suggested that RolA moves laterally on the film.

    Original languageEnglish
    Number of pages1
    Publication statusPublished - 2006 Oct 19
    Event55th SPSJ Annual Meeting - Nagoya, Japan
    Duration: 2006 May 242006 May 26

    Other

    Other55th SPSJ Annual Meeting
    Country/TerritoryJapan
    CityNagoya
    Period06/5/2406/5/26

    Keywords

    • Aspergillus oryzae
    • Cutinase
    • Degradation
    • FRAP
    • Hydrophobin
    • Interaction
    • PBSA
    • QCM

    ASJC Scopus subject areas

    • Engineering(all)

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