The function of rhamnose-binding lectin in innate immunity by restricted binding to Gb3

Yasuharu Watanabe, Hiroaki Tateno, Sachiko Nakamura-Tsuruta, Junko Kominami, Jun Hirabayashi, Osamu Nakamura, Tasuku Watanabe, Hisao Kamiya, Takako Naganuma, Tomohisa Ogawa, Ryno J. Naudé, Koji Muramoto

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64 Citations (Scopus)

Abstract

l-Rhamnose-binding lectins (RBLs) have been isolated from various kinds of fish and invertebrates and interact with various kinds of bacteria, suggesting RBLs are involved in various inflammatory reactions. We investigated the effect of RBLs from chum salmon (Oncorhynchus keta), named CSL1, 2 and 3, on the peritoneal macrophage cell line from rainbow trout (Oncorhynchus mykiss) (RTM5) and an established fibroblastic-like cell line derived from gonadal tissue of rainbow trout (RTG-2). CSLs were bound to the surface of RTM5 and RTG-2 cells and induced proinflammatory cytokines, including IL-1β1, IL-1β2, TNF-α1, TNF-α2 and IL-8 in both cells by recognizing globotriaosylceramide (Gb3). In addition, CSLs had an opsonic effect on RTM5 cells and this effect was significantly inhibited by l-rhamnose, indicating that CSLs enhanced their phagocytosis by binding to Gb3 on cell surfaces. This is the first finding that Gb3 plays a role in innate immunity by cooperating with natural ligands, RBLs.

Original languageEnglish
Pages (from-to)187-197
Number of pages11
JournalDevelopmental and Comparative Immunology
Volume33
Issue number2
DOIs
Publication statusPublished - 2009 Feb 1

Keywords

  • Chum salmon
  • Cytokine
  • Gb3
  • Globotriaosylceramide
  • Innate immunity
  • Lectin
  • Opsonic effect
  • Rhamnose-binding lectin

ASJC Scopus subject areas

  • Immunology
  • Developmental Biology

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  • Cite this

    Watanabe, Y., Tateno, H., Nakamura-Tsuruta, S., Kominami, J., Hirabayashi, J., Nakamura, O., Watanabe, T., Kamiya, H., Naganuma, T., Ogawa, T., Naudé, R. J., & Muramoto, K. (2009). The function of rhamnose-binding lectin in innate immunity by restricted binding to Gb3. Developmental and Comparative Immunology, 33(2), 187-197. https://doi.org/10.1016/j.dci.2008.08.008