TY - JOUR
T1 - The flavoprotein Tah18-dependent NO synthesis confers high-temperature stress tolerance on yeast cells
AU - Nishimura, Akira
AU - Kawahara, Nobuhiro
AU - Takagi, Hiroshi
N1 - Funding Information:
We wish to thank Nobuyuki Yoshida, Iwao Ohtsu, Susumu Morigasaki, and Yu Sasano of our laboratory, Hideo Yamasaki (University of the Ryukyus) and Takaaki Akaike (Kumamoto University) for the discussions on this work. This work was supported by a grant for a Grant-in-Aid for Scientific Research (B) ( 22380061 ) from Japan Society for the Promotion of Science , a Grant-in-Aid for Scientific Research on Innovative Area (ROS Signal) ( 23117711 ), and Global COE Program in NAIST from the Ministry of Education, Science, Culture, Sports and Technology of Japan .
PY - 2013/1/4
Y1 - 2013/1/4
N2 - Nitric oxide (NO) is a ubiquitous signaling molecule involved in the regulation of a large number of cellular functions. In the unicellular eukaryote yeast, NO may be involved in stress response pathways, but its role is poorly understood due to the lack of mammalian NO synthase (NOS) orthologues. Previously, we have proposed the oxidative stress-induced l-arginine synthesis and its physiological role under stress conditions in yeast Saccharomyces cerevisiae. Here, our experimental results indicated that increased conversion of l-proline into l-arginine led to NO production in response to elevated temperature. We also showed that the flavoprotein Tah18, which was previously reported to transfer electrons to the Fe-S cluster protein Dre2, was involved in NO synthesis in yeast. Gene knockdown analysis demonstrated that Tah18-dependent NO synthesis confers high-temperature stress tolerance on yeast cells. As it appears that such a unique cell protection mechanism is specific to yeasts and fungi, it represents a promising target for antifungal activity.
AB - Nitric oxide (NO) is a ubiquitous signaling molecule involved in the regulation of a large number of cellular functions. In the unicellular eukaryote yeast, NO may be involved in stress response pathways, but its role is poorly understood due to the lack of mammalian NO synthase (NOS) orthologues. Previously, we have proposed the oxidative stress-induced l-arginine synthesis and its physiological role under stress conditions in yeast Saccharomyces cerevisiae. Here, our experimental results indicated that increased conversion of l-proline into l-arginine led to NO production in response to elevated temperature. We also showed that the flavoprotein Tah18, which was previously reported to transfer electrons to the Fe-S cluster protein Dre2, was involved in NO synthesis in yeast. Gene knockdown analysis demonstrated that Tah18-dependent NO synthesis confers high-temperature stress tolerance on yeast cells. As it appears that such a unique cell protection mechanism is specific to yeasts and fungi, it represents a promising target for antifungal activity.
KW - High-temperature stress
KW - Nitric oxide
KW - Nitric oxide synthase
KW - Saccharomyces cerevisiae
KW - Tah18
KW - Yeast
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U2 - 10.1016/j.bbrc.2012.11.023
DO - 10.1016/j.bbrc.2012.11.023
M3 - Article
C2 - 23159617
AN - SCOPUS:84872399319
VL - 430
SP - 137
EP - 143
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 1
ER -