The expression of iron homeostasis-related genes during rice germination

Tomoko Nozoye, Haruhiko Inoue, Michiko Takahashi, Yasuhiro Ishimaru, Hiromi Nakanishi, Satoshi Mori, Naoko K. Nishizawa

Research output: Contribution to journalArticlepeer-review

49 Citations (Scopus)

Abstract

To characterize Fe homeostasis during the early stages of seed germination, a microarray analysis was performed. mRNAs extracted from fully mature seeds or seeds harvested 1-3 days after sowing were hybridized to a rice microarray containing approximately 22,000 cDNA oligo probes. Many Fe deficiency-inducible genes were strongly expressed throughout early seed germination. These results suggest that the demand for Fe is extremely high during germination. Under Fe-deficient conditions, rice produces and secretes a metal-cation chelator called deoxymugineic acid (DMA) to acquire Fe from the soil. In addition, DMA and its intermediate nicotianamine (NA) are thought to be involved in long distance Fe transport in rice. Using promoter-β-glucuronidase (GUS) analysis, we investigated the expression patterns during seed germination of the Fe deficiency-inducible genes OsNAS1, OsNAS2, OsNAS3, OsNAAT1, and OsDMAS1, which encode enzymes that participate in the biosynthesis of DMA, and the transporter genes OsYSL2 and OsIRT1, which are involved in Fe transport. All of these genes were expressed in germinating seeds prior to protrusion of the radicle. These results suggest that DMA and NA are produced and involved in Fe transport during germination.

Original languageEnglish
Pages (from-to)35-47
Number of pages13
JournalPlant Molecular Biology
Volume64
Issue number1-2
DOIs
Publication statusPublished - 2007 May
Externally publishedYes

Keywords

  • Microarray analysis
  • Mugineic acid family phytosiderophores
  • Nicotianamine
  • Oryza sativa
  • Seed germination
  • Transporter

ASJC Scopus subject areas

  • Genetics
  • Agronomy and Crop Science
  • Plant Science

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