The Eiken Latex test for detection of a cryptococcal antigen in cryptococcosis - Comparison with a monoclonal antibody-based latex agglutination test, Pastorex® cryptococcus

Ken ichi Tanaka, Shigeru Kohno, Takashige Miyazaki, Haruko Miyazaki, Kotaro Mitsutake, Shigefumi Maesaki, Mitsuo Kaku, Hironobu Koga

Research output: Contribution to journalArticlepeer-review

15 Citations (Scopus)

Abstract

A latex agglutination test for cryptococcal antigen, the Eiken Latex test (Eiken, Tokyo, Japan), was compared with a monoclonal antibody-based agglutination assay, Pastorex® Cryptococcus (Diagnostics Pasteur, Marneur-la-Coquette, France). In a murine model of disseminated cryptococcosis, the kinetics of the antigen titers by the Eiken Latex were similar to those by the Pastorex® Cryptococcus, but sensitivity was much higher. In HIV-negative patients with pulmonary cryptococcosis, a cryptococcal antigen was detected in 6 of 10 patients by the Eiken Latex test and in only 3 of those patients by the Pastorex® Cryptococcus. The results indicate that the Eiken Latex is more sensitive for the detection of the cryptococcal antigen, even in non-disseminated cryptococcosis. The sensitivity and specificity of the Eiken Latex were examined using 195 sera from 25 patients with pulmonary cryptococcosis and 170 patients with non-cryptococcosis. The cutoff value of ≥ 1:8 showed a sensitivity of 76% (19/25) and a specificity of 98.9% (168/170).

Original languageEnglish
Pages (from-to)131-134
Number of pages4
JournalMycopathologia
Volume127
Issue number3
DOIs
Publication statusPublished - 1994 Sep 1

Keywords

  • Cryptococcal antigen
  • Eiken Latex
  • Latex agglutination test
  • Murine disseminated cryptococcosis
  • Pastorex Cryptococcus
  • Pulmonary cryptococcosis

ASJC Scopus subject areas

  • Microbiology
  • Applied Microbiology and Biotechnology
  • Agronomy and Crop Science
  • veterinary (miscalleneous)

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