The effects of G protein modulators on the labellar taste receptor cells of the fleshfly (boettcherisca peregrina)

Masayuki Koganezawa, Ichiro Shimada

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

A nonhydrolyzable G protein activator (guanosine 5'-O-(3-thiotriphosphate); GTPγS) and a G protein inhibitor (guanosine 5'-O-(2-thiodiphosphate); GDPβS) were introduced into the labellar taste receptor cells of the fleshfly by treatment of their receptive membranes beneath the tip opening of the chemosensory hair with each reagent in 0.03% deoxycholate solution for 4 min. After treatment with GTPγS, the responses of the sugar receptor cell to D-glucose, D-fructose, L-phenylalanine and L-valine and that of the salt receptor cell to cyclic AMP were markedly enhanced, compared with those after treatment with deoxycholate alone. Treatment with GDPβS depressed these responses. These results strongly suggest that the responses are mediated by G protein. However, the response of the salt receptor cell to NaCl was not affected by treatment with either GTPγS or GDPβS, and thus the response to NaCl clearly is not elicited through a G protein-regulated mechanism.

Original languageEnglish
Pages (from-to)225-233
Number of pages9
JournalJournal of Insect Physiology
Volume43
Issue number3
DOIs
Publication statusPublished - 1997 Aug 19

Keywords

  • Fly
  • G protein
  • Taste
  • Transduction

ASJC Scopus subject areas

  • Physiology
  • Insect Science

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