The effects of CpG densities around transcription start sites on sex-biased gene expression in Poecilia reticulata

Katsunori Tamagawa, Takashi Makino, Masakado Kawata

    Research output: Contribution to journalArticlepeer-review

    Abstract

    As most genes are shared between females and males, DNA methylation is assumed to play a crucial role in sex-biased gene expression. DNA methylation exclusively occurs at CpG dinucleotides, and therefore, we would expect that CpG density around transcription start sites (TSSs) relate to sex-biased gene expression. Here we investigated the relationship between CpG densities around TSSs and the ratio of gene expression levels between sexes in the guppy (Poecilia reticulata),which displays remarkable sexual dimorphisms.Wefound that geneswithsex-biasedgeneexpressionhaddifferentCpGdensitiesdownstreamof TSSs comparedwith genes lacking sex-biased gene expression. Intriguingly,male-biased expression geneswith intermediateCpGdensity downstream of TSSs exhibited greater differences in gene expression between sexes in the gonad and tail.Our findings suggested the possibility that CpGs around TSSs, especially in the downstream regions, play a crucial role in sex-biased gene expression throughDNAmethylation.

    Original languageEnglish
    Pages (from-to)1204-1211
    Number of pages8
    JournalGenome biology and evolution
    Volume9
    Issue number5
    DOIs
    Publication statusPublished - 2017 May

    Keywords

    • CpG dinucleotides
    • DNA methylation
    • Guppy
    • Sexual dimorphism
    • Transcriptional regulation

    ASJC Scopus subject areas

    • Ecology, Evolution, Behavior and Systematics
    • Genetics

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