The effects of coating culture dishes with collagen on fibroblast cell shape and swirling pattern formation

Kei Hashimoto, Kimiko Yamashita, Kanako Enoyoshi, Xavier Dahan, Tatsu Takeuchi, Hiroshi Kori, Mari Gotoh

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

Motile human-skin fibroblasts form macroscopic swirling patterns when grown to confluence on a culture dish. In this paper, we investigate the effect of coating the culture-dish surface with collagen on the resulting pattern, using human-skin fibroblast NB1RGB cells as the model system. The presence of the collagen coating is expected to enhance the adherence of the fibroblasts to the dish surface, and thereby also enhance the traction that the fibroblasts have as they move. We find that, contrary to our initial expectation, the coating does not significantly affect the motility of the fibroblasts. Their eventual number density at confluence is also unaffected. However, the coherence length of cell orientation in the swirling pattern is diminished. We also find that the fibroblasts cultured in collagen-coated dishes are rounder in shape and shorter in perimeter, compared with those cultured in uncoated polystyrene or glass culture dishes. We hypothesise that the rounder cell-shape which weakens the cell–cell nematic contact interaction is responsible for the change in coherence length. A simple mathematical model of the migrating fibroblasts is constructed, which demonstrates that constant motility with weaker nematic interaction strength does indeed lead to the shortening of the coherence length.

Original languageEnglish
Pages (from-to)351-369
Number of pages19
JournalJournal of Biological Physics
Volume46
Issue number4
DOIs
Publication statusPublished - 2020 Dec

Keywords

  • Cell population
  • Collagen
  • Fibroblast
  • Mathematical model
  • Pattern formation

ASJC Scopus subject areas

  • Biophysics
  • Atomic and Molecular Physics, and Optics
  • Molecular Biology
  • Cell Biology

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