The effects analysis of two neonicotinoid insecticides on in vitro maturation of porcine oocytes using hanging drop monoculture method

Sadamasa Ishikawa, Kou Hiraga, Yuuki Hiradate, Kentaro Tanemura

Research output: Contribution to journalArticle

Abstract

Acetamiprid (ACE) and imidacroprid (IMI) are known neonicotinoid insecticides with strong affinities for the insect-selective nicotinic acetylcholine receptor. These provide insect control by hyperstimulating insect nerves and are used for agricultural pest management. However, it has also been reported that ACE and IMI affect mammalian reproductive function. We determined the effects of ACE and IMI on the in vitro maturation of porcine oocytes. Significant decreases in nuclear maturation rates were observed in the ACE or IMIexposed groups. Also, in matured oocytes from the ACE or IMI-exposed groups, irregular chromosomes were observed. Our results suggest that ACE and IMI exposure was detrimental to porcine oocytes and the extent of the effects depends on the concentration of exposure.

Original languageEnglish
Pages (from-to)725-728
Number of pages4
JournalJournal of Veterinary Medical Science
Volume77
Issue number6
DOIs
Publication statusPublished - 2015 Jul 1

Keywords

  • Hanging drop
  • In vitro maturation (IVM)
  • Neonicotinoid
  • Porcine oocyte

ASJC Scopus subject areas

  • veterinary(all)

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