The effect of the encapsulation of bacteria in redox phospholipid polymer hydrogels on electron transfer efficiency in living cell-based devices

Xiaojie Lin, Koichi Nishio, Tomohiro Konno, Kazuhiko Ishihara

Research output: Contribution to journalArticlepeer-review

42 Citations (Scopus)

Abstract

Development of living cell-based devices holds great promise in many biomedical and industrial applications. To increase our understanding of the process, we investigated the biological and electrochemical properties of a redox phospholipid polymer hydrogel containing an electron-generating bacteria (Shewanella oneidensis MR-1). A water-soluble and amphiphilic phospholipid polymer, poly(2-methacryloyloxyethyl phosphorylcholine-. co-. n-butyl methacrylate-. co-. p-vinylphenylboronic acid-. co-vinylferrocene) (PMBVF), was our choice for incorporation into a hydrogel matrix that promotes encapsulation of bacteria and acts as an electron transfer mediator. This hydrogel formed spontaneously and encapsulated Shewanella in three-dimensional structures. Visual analysis showed that the encapsulated Shewanella maintained viability and metabolic activity even after long-term storage. Cyclic voltammetry measurement indicated that the PMBVF/poly(vinyl alcohol) (PMBVF/PVA) hydrogel had stable and high electron transfer efficiency. Amperometric measurement showed that the hydrogel could maintain the electron transfer efficiency even when Shewanella was encapsulated. Thus, the PMBVF/PVA hydrogel not only provides a mild environment for long-term bacterial survival but also maintains electron transfer efficiency from the bacteria to the electrode. We conclude that hydrogel/bacteria hybrid biomaterials, such as PMBVF/PVA/. Shewanella, may find application in the fabrication of living cell-based devices.

Original languageEnglish
Pages (from-to)8221-8227
Number of pages7
JournalBiomaterials
Volume33
Issue number33
DOIs
Publication statusPublished - 2012 Nov
Externally publishedYes

Keywords

  • 2-Methacryloyloxyethyl phosphorylcholine polymer
  • Cell encapsulation
  • Electron-generating bacteria
  • Microbial-based fuel cell
  • Redox polymer hydrogel

ASJC Scopus subject areas

  • Bioengineering
  • Ceramics and Composites
  • Biophysics
  • Biomaterials
  • Mechanics of Materials

Fingerprint

Dive into the research topics of 'The effect of the encapsulation of bacteria in redox phospholipid polymer hydrogels on electron transfer efficiency in living cell-based devices'. Together they form a unique fingerprint.

Cite this