TY - JOUR
T1 - The effect of the encapsulation of bacteria in redox phospholipid polymer hydrogels on electron transfer efficiency in living cell-based devices
AU - Lin, Xiaojie
AU - Nishio, Koichi
AU - Konno, Tomohiro
AU - Ishihara, Kazuhiko
N1 - Funding Information:
The authors thank Dr. Yuuki Inoue, Mr. Tatsuo Aikawa, and Ms. Kyoko Fukazawa, Department of Materials Engineering, and Dr. Ryuhei Nakamura and Prof. Kazuhito Hashimoto, Department of Applied Chemistry of The University of Tokyo, for their valuable discussions. This research is supported by a Grant-in-Aid for Exploratory Research ( 22650107 ) from the Japan Society for the Promotion of Science (JSPS) and Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency (JST).
PY - 2012/11
Y1 - 2012/11
N2 - Development of living cell-based devices holds great promise in many biomedical and industrial applications. To increase our understanding of the process, we investigated the biological and electrochemical properties of a redox phospholipid polymer hydrogel containing an electron-generating bacteria (Shewanella oneidensis MR-1). A water-soluble and amphiphilic phospholipid polymer, poly(2-methacryloyloxyethyl phosphorylcholine-. co-. n-butyl methacrylate-. co-. p-vinylphenylboronic acid-. co-vinylferrocene) (PMBVF), was our choice for incorporation into a hydrogel matrix that promotes encapsulation of bacteria and acts as an electron transfer mediator. This hydrogel formed spontaneously and encapsulated Shewanella in three-dimensional structures. Visual analysis showed that the encapsulated Shewanella maintained viability and metabolic activity even after long-term storage. Cyclic voltammetry measurement indicated that the PMBVF/poly(vinyl alcohol) (PMBVF/PVA) hydrogel had stable and high electron transfer efficiency. Amperometric measurement showed that the hydrogel could maintain the electron transfer efficiency even when Shewanella was encapsulated. Thus, the PMBVF/PVA hydrogel not only provides a mild environment for long-term bacterial survival but also maintains electron transfer efficiency from the bacteria to the electrode. We conclude that hydrogel/bacteria hybrid biomaterials, such as PMBVF/PVA/. Shewanella, may find application in the fabrication of living cell-based devices.
AB - Development of living cell-based devices holds great promise in many biomedical and industrial applications. To increase our understanding of the process, we investigated the biological and electrochemical properties of a redox phospholipid polymer hydrogel containing an electron-generating bacteria (Shewanella oneidensis MR-1). A water-soluble and amphiphilic phospholipid polymer, poly(2-methacryloyloxyethyl phosphorylcholine-. co-. n-butyl methacrylate-. co-. p-vinylphenylboronic acid-. co-vinylferrocene) (PMBVF), was our choice for incorporation into a hydrogel matrix that promotes encapsulation of bacteria and acts as an electron transfer mediator. This hydrogel formed spontaneously and encapsulated Shewanella in three-dimensional structures. Visual analysis showed that the encapsulated Shewanella maintained viability and metabolic activity even after long-term storage. Cyclic voltammetry measurement indicated that the PMBVF/poly(vinyl alcohol) (PMBVF/PVA) hydrogel had stable and high electron transfer efficiency. Amperometric measurement showed that the hydrogel could maintain the electron transfer efficiency even when Shewanella was encapsulated. Thus, the PMBVF/PVA hydrogel not only provides a mild environment for long-term bacterial survival but also maintains electron transfer efficiency from the bacteria to the electrode. We conclude that hydrogel/bacteria hybrid biomaterials, such as PMBVF/PVA/. Shewanella, may find application in the fabrication of living cell-based devices.
KW - 2-Methacryloyloxyethyl phosphorylcholine polymer
KW - Cell encapsulation
KW - Electron-generating bacteria
KW - Microbial-based fuel cell
KW - Redox polymer hydrogel
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U2 - 10.1016/j.biomaterials.2012.08.035
DO - 10.1016/j.biomaterials.2012.08.035
M3 - Article
C2 - 22938764
AN - SCOPUS:84866143538
VL - 33
SP - 8221
EP - 8227
JO - Biomaterials
JF - Biomaterials
SN - 0142-9612
IS - 33
ER -