Abstract
Development of living cell-based devices holds great promise in many biomedical and industrial applications. To increase our understanding of the process, we investigated the biological and electrochemical properties of a redox phospholipid polymer hydrogel containing an electron-generating bacteria (Shewanella oneidensis MR-1). A water-soluble and amphiphilic phospholipid polymer, poly(2-methacryloyloxyethyl phosphorylcholine-. co-. n-butyl methacrylate-. co-. p-vinylphenylboronic acid-. co-vinylferrocene) (PMBVF), was our choice for incorporation into a hydrogel matrix that promotes encapsulation of bacteria and acts as an electron transfer mediator. This hydrogel formed spontaneously and encapsulated Shewanella in three-dimensional structures. Visual analysis showed that the encapsulated Shewanella maintained viability and metabolic activity even after long-term storage. Cyclic voltammetry measurement indicated that the PMBVF/poly(vinyl alcohol) (PMBVF/PVA) hydrogel had stable and high electron transfer efficiency. Amperometric measurement showed that the hydrogel could maintain the electron transfer efficiency even when Shewanella was encapsulated. Thus, the PMBVF/PVA hydrogel not only provides a mild environment for long-term bacterial survival but also maintains electron transfer efficiency from the bacteria to the electrode. We conclude that hydrogel/bacteria hybrid biomaterials, such as PMBVF/PVA/. Shewanella, may find application in the fabrication of living cell-based devices.
Original language | English |
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Pages (from-to) | 8221-8227 |
Number of pages | 7 |
Journal | Biomaterials |
Volume | 33 |
Issue number | 33 |
DOIs | |
Publication status | Published - 2012 Nov 1 |
Externally published | Yes |
Keywords
- 2-Methacryloyloxyethyl phosphorylcholine polymer
- Cell encapsulation
- Electron-generating bacteria
- Microbial-based fuel cell
- Redox polymer hydrogel
ASJC Scopus subject areas
- Bioengineering
- Ceramics and Composites
- Biophysics
- Biomaterials
- Mechanics of Materials