The effect of the doublesex gene in body colour masculinization of the damselfly Ischnura senegalensis

Michihiko Takahashi, Genta Okude, Ryo Futahashi, Yuma Takahashi, Masakado Kawata

Research output: Contribution to journalArticlepeer-review

Abstract

Odonata species display a remarkable diversity of colour patterns, including intrasexual polymorphisms. In the damselfly (Ischnura senegalensis), the expression of a sex-determining transcription factor, the doublesex (Isdsx) gene is reportedly associated with female colour polymorphism (CP) (gynomorph for female-specific colour and andromorph for male-mimicking colour). Here, the function of Isdsx in thoracic coloration was investigated by electroporation-mediated RNA interference (RNAi). RNAi of the Isdsx common region in males and andromorphic females reduced melanization and thus changed the colour pattern into that of gynomorphic females, while the gynomorphic colour pattern was not affected. By contrast, RNAi against the Isdsx long isoform produced no changes, suggesting that the Isdsx short isoform is important for body colour masculinization in both males and andromorphic females. When examining the expression levels of five genes with differences between sexes and female morphs, two melanin-suppressing genes, black and ebony, were expressed at higher levels in the Isdsx RNAi body area than a control area. Therefore, the Isdsx short isoform may induce thoracic colour differentiation by suppressing black and ebony, thereby generating female CP in I. senegalensis. These findings contribute to the understanding of the molecular and evolutionary mechanisms underlying female CP in Odonata.

Original languageEnglish
Article number20200761
JournalBiology letters
Volume17
Issue number6
DOIs
Publication statusPublished - 2021 Jun 9

Keywords

  • RNA interference
  • damselfly
  • doublesex
  • female-limited polymorphism
  • regulation of gene expression

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Agricultural and Biological Sciences(all)

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