Abstract
The effect of sodium hyaluronate (Na-HA) on the growth of rabbit corneal epithelial cells was investigated in vitro by using MTT assay and [3H]- thymidine incorporation. Na-HA, with a molecular weight of 86 x 104, enhanced the growth of the corneal epithelial cells cultured on a fibronectin (FN) coated dish, but when cells were cultured on a noncoated dish, Na-HA did not enhance the growth of the cells. Na-HA, labeled with 5-aminofluorescein (FA-HA), bound to the corneal epithelial cells, and the CD44-like molecule was identified on the cells by using a monoclonal antibody, OS/37. Furthermore, the binding of FA-HA to the cells was significantly inhibited by the monoclonal antibody. These results suggest that Na-HA binds to the CD44- like molecule associated with FN and then enhances the growth of the corneal epithelial cells.
| Original language | English |
|---|---|
| Pages (from-to) | 409-415 |
| Number of pages | 7 |
| Journal | Journal of Ocular Pharmacology and Therapeutics |
| Volume | 12 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - 1996 |
| Externally published | Yes |
ASJC Scopus subject areas
- Ophthalmology
- Pharmacology
- Pharmacology (medical)