The effect of Cytokines on the Differentiation of an Eosinophilic Leukemia Cell Line (EoL-1) is Associated with Down Regulation of c-myc Gene Expression

Hiroshi Ohtsu, Kohei Yamauchi, Yasuo Tanno, Tamotsu Takishima, Osamu Yoshie, Hiroshi Saito, Norio Hayashi

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

In this study, we measured hydrogen peroxide (H2O2) release as one of the functions of mature eosinophils, and utilized it as a quantitative index. Wedemonstrated that 1) the human eosinophilic leukemia cell line, EoL-1, did not release H2O2 when stimulated with phorbol myristate acetate (PMA), but after culturing with tumor necrosis factor (TNF) and interferon-γ (IFN-γ) it acquired the ability to release H2O2; 2) the ability to release H2O2 was time dependent and reached a peak after 4 days of culture; 3) administration of TGF-β or GM-CSF, with TNF and IFN-γ enhanced the PMA-induced release of H2O2 from EoL-1. To examine the potential relationship between c-myc gene expression and induction of the ability to release H2O2, Northern analysis of c-myc gene expression in EoL-1 cocultured with TNF and IFN-γ was performed. The results showed that the c-myc gene was spontaneously expressed in EoL-1, and the level of c-myc mRNA was markedly reduced after the cells were cocultured with TNFand IFN-γ, suggesting that the decrease of the c-myc mRNA level is closely associated with induction of the ability to release.

Original languageEnglish
Pages (from-to)125-133
Number of pages9
JournalCell structure and function
Volume18
Issue number2
DOIs
Publication statusPublished - 1993

Keywords

  • c-myc
  • eosinophil
  • hydrogen peroxide(H2O2)
  • interferon-γ (IFN-γ)
  • tumor necrosis factor (TNF)

ASJC Scopus subject areas

  • Physiology
  • Molecular Biology
  • Cell Biology

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