The Effect of Atrial Natriuretic Peptide on Cytosolic Free Calcium in Cultured Vascular Smooth Muscle Cells

Kazuhisa Takeuchi, Minoru Yasujima, Makito Sato, Masayuki Kanazawa, Kaoru Yoshinaga, Keishi Abe

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)


Effect of atrial natriuretic peptide (ANP) on cytosolic free calcium ([Ca2+]i) was studied in monolayers of cultured vascular smooth muscle (VSM) cells loaded with a fluorescent calcium indicator, fura-2. Vasoconstrictive hormones, angiotensin II (AII) and Arg8-vasopressin (AVP) induced initial rapid rises in [Ca2+]i, followed by sustained elevation of [Ca2+]i. ANP (Atriopeptin III 10-8M) decreased both the resting level and the sustained elevation of [Ca2+]i induced by AII and AVP. ANP also decreased the rise in [Ca2+]i induced by high potassium (K+) depolarization. AVP-induced initial rapid rise in [Ca2+]i was not inhibited by ANP in the presence or absence of the phosphodiesterase inhibitor, isobutylmethylxanthine 0.1 mM, which has been shown to fully enhance ANP-induced cyclic GMP accumulation. On the other hand, a calcium antagonist, nicardipine, inhibited the high K+-induced rise in [Ca2+]i, whereas it had no effect on not only initial but also sustained rises in [Ca2+]i induced by AVP or AII. These results suggest that ANP has an ability to decrease [Ca2+]i not through inhibition of voltage-sensitive calcium channels, and that neither ANP nor ANP-induced cyclic GMP may affect initial hormone-induced rise in [Ca2+]i. In conclusion, an ability to decrease [Ca2+]i is implicated in ANP-induced relaxation of VSM.

Original languageEnglish
Pages (from-to)47-56
Number of pages10
Journalthe tohoku journal of experimental medicine
Issue number1
Publication statusPublished - 1989


  • atrial natriuretic peptide
  • calcium antagonist
  • cytosolic free calcium
  • vascular smooth muscle cells

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)


Dive into the research topics of 'The Effect of Atrial Natriuretic Peptide on Cytosolic Free Calcium in Cultured Vascular Smooth Muscle Cells'. Together they form a unique fingerprint.

Cite this