The Drosophila mushroom body is a quadruple structure of clonal units each of which contains a virtually identical set of neurones and glial cells

Kei Ito, Wakae Awano, Kazumi Suzuki, Yasushi Hiromi, Daisuke Yamamoto

    Research output: Contribution to journalArticle

    616 Citations (Scopus)

    Abstract

    The mushroom body (MB) is an important centre for higher order sensory integration and learning in insects. To analyse the development and organisation of the MB neuropile in Drosophila, we performed cell lineage analysis in the adult brain with a new technique that combines the Flippase (flp)/FRT system and the GAL4/UAS system. We showed that the four mushroom body neuroblasts (MBNbs) give birth exclusively to the neurones and glial cells of the MB, and that each of the four MBNb clones contributes to the entire MB structure. The expression patterns of 19 GAL4 enhancer-trap strains that mark various subsets of MB cells revealed overlapping cell types in ail four of the MBNb lineages. Partial ablation of MBNbs using hydroxyurea showed that each of the four neuroblasts autonomously generates the entire repertoire of the known MB substructures.

    Original languageEnglish
    Pages (from-to)761-771
    Number of pages11
    JournalDevelopment
    Volume124
    Issue number4
    Publication statusPublished - 1997 Feb 1

    Keywords

    • Brain
    • Cell lineage
    • Development
    • Drosophila
    • Enhancer trap
    • GAL4/UAS
    • Hydroxyurea
    • Mushroom body
    • flp/FRT

    ASJC Scopus subject areas

    • Molecular Biology
    • Developmental Biology

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