The Drosophila Bruton's tyrosine kinase (Btk) homolog is required for adult survival and male genital formation

Kotaro Baba, Aya Takeshita, Kei Majima, Ryu Ueda, Shunzo Kondo, Naoto Juni, Daisuke Yamamoto

    Research output: Contribution to journalArticle

    43 Citations (Scopus)

    Abstract

    We isolated a Drosophila fickle(P) (fic(P)) mutant with a shortened copulatory duration and reduced adult-stage life span. The reduced copulatory duration is ascribable to incomplete fusion of the left and right halves of the apodeme that holds the penis during copulation. fic(P) is an intronic mutation occurring in the Btk gene, a gene which encodes two forms (type 1 and type 2) of a Bruton's tyrosine kinase (Btk) family cytoplasmic tyrosine kinase as a result of alternative exon usage. The fic(P) mutation prevents the formation of the type 2 isoform but leaves expression of the type 1 transcript intact. Ubiquitous overexpression of the wild-type cDNA by using a heat shock 70 promoter during the late larval or pupal stages rescued the life span and genital defects in the mutant, respectively, establishing the causal relationship between the fic(P) phenotypes and the Btk gene mutation. The stage specificity of the rescuing ability suggests that the Btk gene is required for the development of male genitalia and substrates required for adult survival.

    Original languageEnglish
    Pages (from-to)4405-4413
    Number of pages9
    JournalMolecular and cellular biology
    Volume19
    Issue number6
    DOIs
    Publication statusPublished - 1999 Jun

    ASJC Scopus subject areas

    • Molecular Biology
    • Cell Biology

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