The Drosophila Bruton's tyrosine kinase (Btk) homolog is required for adult survival and male genital formation

Kotaro Baba, Aya Takeshita, Kei Majima, Ryu Ueda, Shunzo Kondo, Naoto Juni, Daisuke Yamamoto

Research output: Contribution to journalArticlepeer-review

45 Citations (Scopus)

Abstract

We isolated a Drosophila fickle(P) (fic(P)) mutant with a shortened copulatory duration and reduced adult-stage life span. The reduced copulatory duration is ascribable to incomplete fusion of the left and right halves of the apodeme that holds the penis during copulation. fic(P) is an intronic mutation occurring in the Btk gene, a gene which encodes two forms (type 1 and type 2) of a Bruton's tyrosine kinase (Btk) family cytoplasmic tyrosine kinase as a result of alternative exon usage. The fic(P) mutation prevents the formation of the type 2 isoform but leaves expression of the type 1 transcript intact. Ubiquitous overexpression of the wild-type cDNA by using a heat shock 70 promoter during the late larval or pupal stages rescued the life span and genital defects in the mutant, respectively, establishing the causal relationship between the fic(P) phenotypes and the Btk gene mutation. The stage specificity of the rescuing ability suggests that the Btk gene is required for the development of male genitalia and substrates required for adult survival.

Original languageEnglish
Pages (from-to)4405-4413
Number of pages9
JournalMolecular and cellular biology
Volume19
Issue number6
DOIs
Publication statusPublished - 1999 Jun
Externally publishedYes

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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