The discovery of shorter synthetic proteolytic peptides derived from Tob1 protein

Rina Nakamura, Motomi Konishi, Masanari Taniguchi, Yusuke Hatakawa, Toshifumi Akizawa

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

We screened nearly 1000 synthetic peptides and found that JAL-AK22 (KYEGHWYPEKPYKGSGFRCIHI), which is derived from the BoxA domain in the Tob1 protein, activates both unfolded and folded proMMP-7. Interestingly, the smaller derivative of JAL-AK22, termed JAL-TA9 (YKGSGFRMI)possessed auto-proteolytic activity and cleaved three synthetic peptides fragment (MMP18-33, MMP18-40, and Aβ11-29)under physiological conditions. The kcat of JAL-TA9 was 4.58 × 10−4 min−1 against MMP18-33 and 6.5 × 10−4 min−1 against MMP18-40. These kinetic parameters are lower than those of general proteinases like trypsin, for which the kcat is 247.2 × 105 min−1 against benzoyl-L-arginine ethyl ester. In addition, a 5-mer peptide derived from JAL-TA9, GSGFR also cleaved Aβ11-29. These proteolytic activities were inhibited by AEBSF (4-(2-Aminoethyl)benzenesulfonyl fluoride hydrochloride), a serine protease inhibitor. Our results demonstrate that some small synthetic peptides have protease activity. Thus, we propose calling small peptides possessing with protease activity Catalytides (catalytic peptides). We expect that our findings will stimulate the development of novel Catalytides and related applications such as the development of strategic peptide drugs.

Original languageEnglish
Pages (from-to)71-77
Number of pages7
JournalPeptides
Volume116
DOIs
Publication statusPublished - 2019 Jun
Externally publishedYes

Keywords

  • Catalytide
  • Serine protease-like peptide
  • Tob1

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Endocrinology
  • Cellular and Molecular Neuroscience

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