The developmental changes of mRNA levels for a cerebellar protein (spot 35 protein) in rat brains

T. Yamakuni, K. Araki, Y. Takahashi

Research output: Contribution to journalArticlepeer-review

29 Citations (Scopus)

Abstract

Our previous papers described a protein called spot 35 found in the cerebellar cytosol of adult rats by two-dimensional gel electrophoresis and localized in the Purkinje cells by immunohistochemical methods. Here we describe the biosynthesis of this spot 35 protein using a reticulocyte lysate cell-free system containing rat cerebellar mRNA. The developmental changes of mRNA-dependent protein biosynthesis were also examined. During postnatal 10-30 days, a rapid increase of mRNA levels for spot 35 protein was observed. The application of the new 45Ca-binding assay procedure revealed that this protein is a Ca-binding protein.

Original languageEnglish
Pages (from-to)127-130
Number of pages4
JournalFEBS Letters
Volume188
Issue number1
DOIs
Publication statusPublished - 1985 Aug 19

Keywords

  • Brain development
  • Cerebellar protein
  • Translation
  • mRNA

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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