The determination of multiple microsatellite genotypes and DNA sequences from a single pollen grain: Technical article

Yu Matsuki, Y. Isagi, Y. Suyama

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Pollen plays important roles in the reproduction and gene flow of flowering plants, and its haploid DNA sequence provides useful information for studies of plant evolution and genealogy. We describe a new method for multiple microsatellite genotyping and DNA sequencing from a single pollen grain. The haploid DNA was extracted from a single pollen grain by using a simple DNA extraction method, and multiple microsatellite genotypes and DNA sequences of multiple chloroplast loci were determined. Using nine pairs of microsatellite primers, more than 90% of genotypes were successfully determined, and 71% and 100% of DNA sequences were determined at two chloroplast DNA loci, the trnL intron region and the trnL/trnF intergenic spacer region, respectively. This simple method of genetic analysis for a single pollen grain will facilitate detailed study of pollination, evolution and genealogy.

Original languageEnglish
Pages (from-to)194-198
Number of pages5
JournalMolecular Ecology Notes
Volume7
Issue number2
DOIs
Publication statusPublished - 2007 Mar 1

Keywords

  • Chloroplast DNA
  • DNA sequencing
  • Microsatellite
  • Multiplex PCR
  • Single pollen grain
  • Whole genome amplification

ASJC Scopus subject areas

  • Biochemistry
  • Ecology
  • Biochemistry, Genetics and Molecular Biology(all)

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