The D3 F-box protein is a key component in host strigolactone responses essential for arbuscular mycorrhizal symbiosis

Satoko Yoshida, Hiromu Kameoka, Misaki Tempo, Kohki Akiyama, Mikihisa Umehara, Shinjiro Yamaguchi, Hideo Hayashi, Junko Kyozuka, Ken Shirasu

    Research output: Contribution to journalArticle

    78 Citations (Scopus)

    Abstract

    Arbuscular mycorrhiza (AM) represents an ancient endosymbiosis between plant roots and Glomeromycota fungi. Strigolactones (SLs), plant-derived terpenoid lactones, activate hyphal branching of AM fungi before physical contact. Lack of SL biosynthesis results in lower colonization of AM fungi. The F-box protein, DWARF3 (D3), and the hydrolase family protein DWARF14 (D14) are crucial for SL responses in rice. Here we conducted AM fungal colonization assays with the SL-insensitive d3 and d14 mutants. The d3 mutant exhibited strong defects in AM fungal colonization, whereas the d14 mutant showed higher AM fungal colonization. As D14 has a homologous protein, D14-LIKE, we generated D14-LIKE knockdown lines by RNA interference in the wildtype and d14 background. D14 and D14-LIKE double knockdown lines exhibited similar colonization rates as those of the d14-1 mutant. D3 is crucial for establishing AM symbiosis in rice, whereas D14 and D14-LIKE are not. Our results suggest distinct roles for these SL-related components in AM symbiosis.

    Original languageEnglish
    Pages (from-to)1208-1216
    Number of pages9
    JournalNew Phytologist
    Volume196
    Issue number4
    DOIs
    Publication statusPublished - 2012 Dec 1

    Keywords

    • Arbuscular mycorrhiza
    • F-box protein
    • Rice
    • Strigolactone
    • Tiller outgrowth

    ASJC Scopus subject areas

    • Physiology
    • Plant Science

    Fingerprint Dive into the research topics of 'The D3 F-box protein is a key component in host strigolactone responses essential for arbuscular mycorrhizal symbiosis'. Together they form a unique fingerprint.

  • Cite this

    Yoshida, S., Kameoka, H., Tempo, M., Akiyama, K., Umehara, M., Yamaguchi, S., Hayashi, H., Kyozuka, J., & Shirasu, K. (2012). The D3 F-box protein is a key component in host strigolactone responses essential for arbuscular mycorrhizal symbiosis. New Phytologist, 196(4), 1208-1216. https://doi.org/10.1111/j.1469-8137.2012.04339.x