The D3 F-box protein is a key component in host strigolactone responses essential for arbuscular mycorrhizal symbiosis

Satoko Yoshida, Hiromu Kameoka, Misaki Tempo, Kohki Akiyama, Mikihisa Umehara, Shinjiro Yamaguchi, Hideo Hayashi, Junko Kyozuka, Ken Shirasu

Research output: Contribution to journalArticlepeer-review

95 Citations (Scopus)

Abstract

Arbuscular mycorrhiza (AM) represents an ancient endosymbiosis between plant roots and Glomeromycota fungi. Strigolactones (SLs), plant-derived terpenoid lactones, activate hyphal branching of AM fungi before physical contact. Lack of SL biosynthesis results in lower colonization of AM fungi. The F-box protein, DWARF3 (D3), and the hydrolase family protein DWARF14 (D14) are crucial for SL responses in rice. Here we conducted AM fungal colonization assays with the SL-insensitive d3 and d14 mutants. The d3 mutant exhibited strong defects in AM fungal colonization, whereas the d14 mutant showed higher AM fungal colonization. As D14 has a homologous protein, D14-LIKE, we generated D14-LIKE knockdown lines by RNA interference in the wildtype and d14 background. D14 and D14-LIKE double knockdown lines exhibited similar colonization rates as those of the d14-1 mutant. D3 is crucial for establishing AM symbiosis in rice, whereas D14 and D14-LIKE are not. Our results suggest distinct roles for these SL-related components in AM symbiosis.

Original languageEnglish
Pages (from-to)1208-1216
Number of pages9
JournalNew Phytologist
Volume196
Issue number4
DOIs
Publication statusPublished - 2012 Dec

Keywords

  • Arbuscular mycorrhiza
  • F-box protein
  • Rice
  • Strigolactone
  • Tiller outgrowth

ASJC Scopus subject areas

  • Physiology
  • Plant Science

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