The cytoplasmic tail of heparin-binding EGF-like growth factor regulates bidirectional intracellular trafficking between the plasma membrane and ER

Miki Hieda, Michiko Koizumi, Chiduru Higashi, Taro Tachibana, Tomohiko Taguchi, Shigeki Higashiyama

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

Heparin-binding epidermal growth factor (EGF). - like growth factor (HB-EGF) is synthesized in the ER, transported along the exocytic pathway, and expressed on the plasma membrane as a type I transmembrane protein. Upon extracellular stimulation, HB-EGF, either proHB-EGF or the shed form HB-EGF-CTF, undergoes endocytosis and is then transported retrogradely to the ER. In this study, we showed the essential contribution of the short cytoplasmic tail of HB-EGF (HB-EGF-cyto) to the bidirectional intracellular trafficking between the ER and plasma membrane and revealed several critical amino acids residues that are responsible for internalization from the plasma membrane and ER targeting. We suggest that these anterograde and retrograde sorting signals within HB-EGF-cyto are strictly regulated by protein modification and conformation.

Original languageEnglish
Pages (from-to)339-344
Number of pages6
JournalFEBS Open Bio
Volume2
DOIs
Publication statusPublished - 2012

Keywords

  • ER retrieval
  • Ectodomain shedding
  • HB-EGF
  • Intracellular trafficking

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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