TY - JOUR
T1 - The C2H2-type transcription factor, FlbC, is involved in the transcriptional regulation of Aspergillus oryzae glucoamylase and protease genes specifically expressed in solid-state culture
AU - Tanaka, Mizuki
AU - Yoshimura, Midori
AU - Ogawa, Masahiro
AU - Koyama, Yasuji
AU - Shintani, Takahiro
AU - Gomi, Katsuya
N1 - Funding Information:
We are grateful to Yoji Hata for kindly providing the anti-GlaB antibody and Yukihiro Nakamura for technical advice about the tyrosinase activity assay. We also thank Takeshi Akao for technical advice about total RNA extraction from A. oryzae cultured in solid-state culture and Osamu Mizutani for kindly providing the ∆ligD::loxP pyrG strain. This study was supported by the Program for Promotion of Basic and Applied Researches for Innovations in Bio-oriented Industry and the Science and Technology Research Promotion Program for Agriculture, Forestry, Fisheries and Food Industry. −
Publisher Copyright:
© 2016, Springer-Verlag Berlin Heidelberg.
PY - 2016/7/1
Y1 - 2016/7/1
N2 - Aspergillus oryzae produces a large amount of secreted proteins in solid-state culture, and some proteins such as glucoamylase (GlaB) and acid protease (PepA) are specifically produced in solid-state culture, but rarely in submerged culture. From the disruption mutant library of A. oryzae transcriptional regulators, we successfully identified a disruption mutant showing an extremely low production level of GlaB but a normal level of α-amylase production. This strain was a disruption mutant of the C2H2-type transcription factor, FlbC, which is reported to be involved in the regulation of conidiospore development. Disruption mutants of other upstream regulators comprising a conidiation regulatory network had no apparent effect on GlaB production in solid-state culture. In addition to GlaB, the production of acid protease in solid-state culture was also markedly decreased by flbC disruption. Northern blot analyses revealed that transcripts of glaB and pepA were significantly decreased in the flbC disruption strain. These results suggested that FlbC is involved in the transcriptional regulation of genes specifically expressed under solid-state cultivation conditions, possibly independent of the conidiation regulatory network.
AB - Aspergillus oryzae produces a large amount of secreted proteins in solid-state culture, and some proteins such as glucoamylase (GlaB) and acid protease (PepA) are specifically produced in solid-state culture, but rarely in submerged culture. From the disruption mutant library of A. oryzae transcriptional regulators, we successfully identified a disruption mutant showing an extremely low production level of GlaB but a normal level of α-amylase production. This strain was a disruption mutant of the C2H2-type transcription factor, FlbC, which is reported to be involved in the regulation of conidiospore development. Disruption mutants of other upstream regulators comprising a conidiation regulatory network had no apparent effect on GlaB production in solid-state culture. In addition to GlaB, the production of acid protease in solid-state culture was also markedly decreased by flbC disruption. Northern blot analyses revealed that transcripts of glaB and pepA were significantly decreased in the flbC disruption strain. These results suggested that FlbC is involved in the transcriptional regulation of genes specifically expressed under solid-state cultivation conditions, possibly independent of the conidiation regulatory network.
KW - Acid protease
KW - Aspergillus oryzae
KW - Conidiospore development
KW - Gene expression regulation
KW - Glucoamylase
KW - Solid-state culture
KW - Transcription factor FlbC
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U2 - 10.1007/s00253-016-7419-6
DO - 10.1007/s00253-016-7419-6
M3 - Article
C2 - 26960315
AN - SCOPUS:84960085043
VL - 100
SP - 5859
EP - 5868
JO - Applied Microbiology and Biotechnology
JF - Applied Microbiology and Biotechnology
SN - 0175-7598
IS - 13
ER -