The conserved oligomeric Golgi complex is involved in double-membrane vesicle formation during autophagy

Wei Lien Yen, Takahiro Shintani, Usha Nair, Yang Cao, Brian C. Richardson, Zhijian Li, Frederick M. Hughson, Misuzu Baba, Daniel J. Klionsky

Research output: Contribution to journalArticle

143 Citations (Scopus)

Abstract

Macroautophagy is a catabolic pathway used for the turnover of long-lived proteins and organelles in eukaryotic cells. The morphological hallmark of this process is the formation of doublemembrane autophagosomes that sequester cytoplasm. Autophagosome formation is the most complex part of macroautophagy, and it is a dynamic event that likely involves vesicle fusion to expand the initial sequestering membrane, the phagophore; however, essentially nothing is known about this process including the molecular components involved in vesicle tethering and fusion. In this study, we provide evidence that the subunits of the conserved oligomeric Golgi (COG) complex are required for double-membrane cytoplasm to vacuole targeting vesicle and autophagosome formation. COG subunits localized to the phagophore assembly site and interacted with Atg (autophagy related) proteins. In addition, mutations in the COG genes resulted in the mislocalization of Atg8 and Atg9, which are critical components involved in autophagosome formation.

Original languageEnglish
Pages (from-to)101-114
Number of pages14
JournalJournal of Cell Biology
Volume188
Issue number1
DOIs
Publication statusPublished - 2010 Dec 11

ASJC Scopus subject areas

  • Cell Biology

Fingerprint Dive into the research topics of 'The conserved oligomeric Golgi complex is involved in double-membrane vesicle formation during autophagy'. Together they form a unique fingerprint.

  • Cite this

    Yen, W. L., Shintani, T., Nair, U., Cao, Y., Richardson, B. C., Li, Z., Hughson, F. M., Baba, M., & Klionsky, D. J. (2010). The conserved oligomeric Golgi complex is involved in double-membrane vesicle formation during autophagy. Journal of Cell Biology, 188(1), 101-114. https://doi.org/10.1083/jcb.200904075