The Cl- effect on photosynthetic oxygen evolution: interaction of Cl- with 18-kDa, 24-kDa and 33-kDa proteins

Mitsue Miyao, Norio Murata

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130 Citations (Scopus)


The interaction of Cl- with the extrinsic proteins of 18 kDa, 24 kDa and 33 kDa in the photosynthetic oxygen-evolution complex was studied by comparing spinach photosystem II particles of different protein compositions. The 33-kDa protein decreased the Cl- concentration optimum for oxygen evolution from 150 to 30 mM, and the 24-kDa protein decreased it from 30 to 10 mM. The 18-kDa protein did not change the optimum Cl- concentration, but sustained oxygen evolution at Cl- concentrations lower than 3 mM. The presence of the 24-kDa and 18-kDa proteins, but not each protein alone, markedly suppressed inactivation of oxygen evolution at a very low Cl- concentration and its restoration by readdition of Cl-.

Original languageEnglish
Pages (from-to)303-308
Number of pages6
JournalFEBS Letters
Issue number2
Publication statusPublished - 1985 Jan 28
Externally publishedYes


  • (Spinach chloroplast)
  • Cl effect
  • Oxygen evolution
  • Photosynthesis
  • Photosystem II

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology


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