The cloning of PIG-A, a component in the early step of GPI-anchor biosynthesis

Toshio Miyata; Junji Takeda; Yoshiyasu Iida; Norio Yamada; Norimitsu Inoue; Minoru Takahashi; Kenji Maeda; Teruo Kitani; Taroh Kinoshita

doi:10.1126/science.7680492

The cloning of PIG-A, a component in the early step of GPI-anchor biosynthesis

Toshio Miyata, Junji Takeda, Yoshiyasu Iida, Norio Yamada, Norimitsu Inoue, Minoru Takahashi, Kenji Maeda, Teruo Kitani, Taroh Kinoshita

Research output: Contribution to journal › Article › peer-review

408 Citations (Scopus)

Abstract

The glycosylphosphatidylinositol (GPI) anchor is a membrane attachment structure of many proteins and occurs in a wide variety of eukaryotes from yeasts to mammals. The structure of the core of the GPI anchor is conserved in protozoa and mammals and so is its biosynthetic pathway. A complementary DNA encoding a human protein termed PIG-A (phosphatidylinositol glycan-class A) was cloned. PIG-A was necessary for synthesis of N-acetylglucosaminyl- phosphatidylinositol, the very early intermediate in GPI-anchor biosynthesis.

Original language	English
Pages (from-to)	1318-1320
Number of pages	3
Journal	Science
Volume	259
Issue number	5099
DOIs	https://doi.org/10.1126/science.7680492
Publication status	Published - 1993 Jan 1
Externally published	Yes

ASJC Scopus subject areas

General

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AU - Takeda, Junji

AU - Iida, Yoshiyasu

AU - Yamada, Norio

AU - Inoue, Norimitsu

AU - Takahashi, Minoru

AU - Maeda, Kenji

AU - Kitani, Teruo

AU - Kinoshita, Taroh

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