The chimeric repressor for the GATA4 transcription factor improves tolerance to nitrogen deficiency in Arabidopsis

Ji Min Shin, Kwi Mi Chung, Shingo Sakamoto, Soichi Kojima, Chuan Ming Yeh, Miho Ikeda, Nobutaka Mitsuda, Masaru Ohme-Takagi

    Research output: Contribution to journalArticlepeer-review

    7 Citations (Scopus)

    Abstract

    Nitrogen limits crop yield, but application of nitrogen fertilizer can cause environmental problems and much fertilizer is lost without being absorbed by plants. Increasing nitrogen use efficiency in plants may help overcome these problems and is, therefore, an important and active subject of agricultural research. Here, we report that the expression of the chimeric repressor for the GATA4 transcription factor (35S:GATA4-SRDX) improved tolerance to nitrogen deficiency in Arabidopsis thaliana. 35S:GATA4-SRDX seedlings were significantly larger than wild type under nitrogensufficient and -deficient conditions (10 and 0.5 mM NH4NO3, respectively). 35S:GATA4-SRDX plants exhibited shorter primary roots, fewer lateral roots, and higher root hair density compared with wild type. The expression levels of NITRATE TRANSPORTER 2.1, ASPARAGINE SYNTHETASE and NITRATE REDUCTASE 1 were significantly higher in roots of 35S:GATA4-SRDX plants than in wild type under nitrogen-sufficient conditions. Under nitrogen-deficient conditions, the expression of genes for cytosolic glutamine synthetases was upregulated in shoots of 35S:GATA4-SRDX plants compared with wild type. This upregulation of nitrogen transporter and nitrogen assimilation-related genes might confer tolerance to nitrogen deficiency in 35S:GATA4-SRDX plants.

    Original languageEnglish
    Pages (from-to)151-158
    Number of pages8
    JournalPlant Biotechnology
    Volume34
    Issue number3
    DOIs
    Publication statusPublished - 2017

    Keywords

    • Arabidopsis
    • Chimeric repressor
    • Nitrogen use efficiency
    • Tolerance
    • Transcription factor

    ASJC Scopus subject areas

    • Biotechnology
    • Agronomy and Crop Science
    • Plant Science

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