The cDNA cloning and transient expression of a chicken gene encoding cytochrome P-450scc

Osamu Nomura, Osamu Nakabayashi, Katsuhiko Nishimori, Shigeki Mizuno

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

A cDNA clone, pcSCC1, isolated from a chicken adrenal gland library showed about 62% nucleotide sequence similarity to those of mammalian and rainbow trout P-450scc cDNAs and was proved to be the clone for chicken P-450scc by the enzymatic activity of converting 25-hydroxycholesterol to pregnenolone demonstrated in the transfected COS-7 cells. Comparison of deduced amino acid sequences shows significant conservation of heme- and steroid-binding domains but little similarity in the putative N-terminal presequence region among chicken, trout and mammals. The chicken cDNA lacks a polyadenylation signal in its 3'-UTR. Sequencing of a 3' end region of the P-450scc gene (CYP11A1) suggests that a region containing the polyadenylation signal is spliced out from the pre-mRNA.

Original languageEnglish
Pages (from-to)217-222
Number of pages6
JournalGene
Volume185
Issue number2
DOIs
Publication statusPublished - 1997 Feb 7

Keywords

  • Conserved sequence region
  • Enzyme assay
  • Splicing out of polyadenylation signal
  • Transfection

ASJC Scopus subject areas

  • Genetics

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