The cbbL gene is required for thiosulfate-dependent autotrophic growth of Bradyrhizobium japonicum

Sachiko Masuda, Shima Eda, Chiaki Sugawara, Hisayuki Mitsui, Kiwamu Minamisawa

    Research output: Contribution to journalArticlepeer-review

    18 Citations (Scopus)

    Abstract

    Bradyrhizobium japonicum is a facultative chemolithoautotroph capable of using thiosulfate and H2 as an electron donor and CO2 as a carbon source. In B. japonicum USDA110, the mutant of cbbL gene encoding a large subunit of ribulose 1,5-bisphosphate carboxylase/oxygenase (RuBisCO) was unable to grow using thiosulfate and H2 as an electron donor. The cbbL deletion mutant was able to grow and oxidize thiosulfate in the presence of succinate. These results showed that the major route of CO2 fixation for thiosulfate-dependent chemoautotrophic growth is the Calvin-Benson-Bassham cycle involving RuBisCO in B. japonicum.

    Original languageEnglish
    Pages (from-to)220-223
    Number of pages4
    JournalMicrobes and environments
    Volume25
    Issue number3
    DOIs
    Publication statusPublished - 2010

    Keywords

    • Bradyrhizobium japonicum
    • CO
    • Chemoautotroph
    • Fixation
    • cbb

    ASJC Scopus subject areas

    • Ecology, Evolution, Behavior and Systematics
    • Soil Science
    • Plant Science

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