The canonical Wnt pathway directly regulates NRSF/REST expression in chick spinal cord

Shigeki Nishihara, Leo Tsuda, Toshihiko Ogura

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

Neural precursor cells actively proliferate in the ventricular zone to self-renew the stem cell population, and in parallel, maintain their undifferentiated state. This progenitor pool generates postmitotic cells that migrate to the mantle layer and differentiate into mature neurons. The growth of these stem cells is strictly controlled by the canonical Wnt signaling cascade, in part mediated by the direct regulation of Cyclin D1, a critical regulator of cell cycle progression. Here, we report that the canonical Wnt pathway directly controls the expression of NRSF/REST. The Wnt-activated β-catenin/TCF complex up-regulates this gene through a conserved element found in its exon 1a, a critical result obtained by a novel in ovo transcriptional assay. Hence, our data show that the canonical Wnt signaling cascade directly regulates the NRSF/REST and Cyclin D1 genes, thereby controlling the progenitor cells. In addition, we show that our in ovo transcription assay is a powerful way to analyze gene regulation in a natural in vivo context.

Original languageEnglish
Pages (from-to)55-63
Number of pages9
JournalBiochemical and biophysical research communications
Volume311
Issue number1
DOIs
Publication statusPublished - 2003 Nov 7

Keywords

  • Canonical Wnt pathway
  • In ovo electroporation
  • NRSF/REST
  • Progenitor cells
  • Spinal cord
  • Wnt1
  • β-catenin

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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