TY - JOUR
T1 - The canonical Wnt pathway directly regulates NRSF/REST expression in chick spinal cord
AU - Nishihara, Shigeki
AU - Tsuda, Leo
AU - Ogura, Toshihiko
N1 - Funding Information:
We thank Drs. H. Shibuya, S. Nakagawa, M. Wassef, and S. Kitazawa for plasmids and probes. This work was supported by a Grant-in-Aid for Scientific Research on Priority Areas (C) from the Ministry of Education, Science, Sports and Culture of Japan (T.O.), and a Creative Basic Research grant from the Ministry of Education, Science, Sports and Culture of Japan (T.O.).
PY - 2003/11/7
Y1 - 2003/11/7
N2 - Neural precursor cells actively proliferate in the ventricular zone to self-renew the stem cell population, and in parallel, maintain their undifferentiated state. This progenitor pool generates postmitotic cells that migrate to the mantle layer and differentiate into mature neurons. The growth of these stem cells is strictly controlled by the canonical Wnt signaling cascade, in part mediated by the direct regulation of Cyclin D1, a critical regulator of cell cycle progression. Here, we report that the canonical Wnt pathway directly controls the expression of NRSF/REST. The Wnt-activated β-catenin/TCF complex up-regulates this gene through a conserved element found in its exon 1a, a critical result obtained by a novel in ovo transcriptional assay. Hence, our data show that the canonical Wnt signaling cascade directly regulates the NRSF/REST and Cyclin D1 genes, thereby controlling the progenitor cells. In addition, we show that our in ovo transcription assay is a powerful way to analyze gene regulation in a natural in vivo context.
AB - Neural precursor cells actively proliferate in the ventricular zone to self-renew the stem cell population, and in parallel, maintain their undifferentiated state. This progenitor pool generates postmitotic cells that migrate to the mantle layer and differentiate into mature neurons. The growth of these stem cells is strictly controlled by the canonical Wnt signaling cascade, in part mediated by the direct regulation of Cyclin D1, a critical regulator of cell cycle progression. Here, we report that the canonical Wnt pathway directly controls the expression of NRSF/REST. The Wnt-activated β-catenin/TCF complex up-regulates this gene through a conserved element found in its exon 1a, a critical result obtained by a novel in ovo transcriptional assay. Hence, our data show that the canonical Wnt signaling cascade directly regulates the NRSF/REST and Cyclin D1 genes, thereby controlling the progenitor cells. In addition, we show that our in ovo transcription assay is a powerful way to analyze gene regulation in a natural in vivo context.
KW - Canonical Wnt pathway
KW - In ovo electroporation
KW - NRSF/REST
KW - Progenitor cells
KW - Spinal cord
KW - Wnt1
KW - β-catenin
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U2 - 10.1016/j.bbrc.2003.09.158
DO - 10.1016/j.bbrc.2003.09.158
M3 - Article
C2 - 14575694
AN - SCOPUS:0142059234
VL - 311
SP - 55
EP - 63
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 1
ER -