The Canoe protein is necessary in adherens junctions for development of ommatidial architecture in the Drosophila compound eye

Takashi Matsuo, Kuniaki Takahashi, Emiko Suzuki, Daisuke Yamamoto

    Research output: Contribution to journalArticlepeer-review

    33 Citations (Scopus)

    Abstract

    Rhabdomeres of the Drosophila melanogaster canoe(misl) mutant ommatidia were twisted, branched, and often fused to each other. A considerable proportion of rhabdomeres were found to have fallen below the retinal basement membrane. Electron-microscopic observations of the mutant ommatidia revealed that microvilli, the subcellular structures composing the rhabdome, were normal. As was the case with partial loss-of-function mutations in the canoe locus, overexpression of the wild-type canoe transgene driven by the heat shock promoter or sevenless enhancer in the wild-type canoe background caused malformation of the rhabdomeres in the adult ommatidia. Immunolabeling of the Canoe protein in the pupal retinae showed that it was accumulated in adherens junctions in photoreceptor rhabdomeres at high concentrations, as well as in pigment cells, bristle cells, and the interjunctional region of photoreceptors at a lower level. In the canoe mutant ommatidia, the Canoe protein concentration was dramatically decreased in adherens junctions, while it was maintained at a level comparable with the wild-type flies in the interjunctional region. Since Canoe or its mammalian homolog AF-6 is known to bind to F-actin and Ras, we suggest the possibility that Canoe couples Ras signaling with cytoskeleton, thereby supporting the straight elongation of rhabdomere required for development of a regular array of ommatidia.

    Original languageEnglish
    Pages (from-to)397-404
    Number of pages8
    JournalCell and Tissue Research
    Volume298
    Issue number3
    DOIs
    Publication statusPublished - 1999 Dec 17

    Keywords

    • Development
    • Drosophila melanogaster (Insecta)
    • Eye mutants
    • PDZ-domain protein
    • Photoreceptor
    • Rhabdomere

    ASJC Scopus subject areas

    • Pathology and Forensic Medicine
    • Histology
    • Cell Biology

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