The Caenorhabditis elegans DDX-23, a homolog of yeast splicing factor PRP28, is required for the sperm-oocyte switch and differentiation of various cell types

Takafumi Konishi, Nobuko Uodome, Asako Sugimoto

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

DEAD/H-box proteins are involved in various aspects of RNA metabolism. Here we report the developmental function of a DEAD-box protein, DDX-23, in Caenorhabditis elegans, which has significant homology with the yeast splicing factor PRP28. We found by RNAi and mutant analyses that DDX-23 is essential for both embryonic and post-embryonic development, and required for differentiation of the majority of somatic tissues. When the germline function of ddx-23 was inhibited, hermaphrodite animals showed a reduced number of germ cells and failed to switch from spermatogenesis to oogenesis. These phenotypes were similar to those of the mutants of the three DEAH-box proteins (MOG-1, MOG-4, and MOG-5) whose yeast orthologs are involved in the pre-mRNA splicing pathway. We speculate that DDX-23 functions with the three MOG proteins in the same pathway to regulate tissue differentiation, robust germline proliferation, and the sperm/oocyte switch through modulations of ribonucleoprotein complexes.

Original languageEnglish
Pages (from-to)2367-2377
Number of pages11
JournalDevelopmental Dynamics
Volume237
Issue number9
DOIs
Publication statusPublished - 2008 Sep 1
Externally publishedYes

Keywords

  • C. elegans
  • DEAD/H-box proteins
  • Differentiation
  • Gametogenesis
  • Splicing

ASJC Scopus subject areas

  • Developmental Biology

Fingerprint Dive into the research topics of 'The Caenorhabditis elegans DDX-23, a homolog of yeast splicing factor PRP28, is required for the sperm-oocyte switch and differentiation of various cell types'. Together they form a unique fingerprint.

  • Cite this