The C. elegans eyes absent ortholog EYA-1 is required for tissue differentiation and plays partially redundant roles with PAX-6

Miwa Furuya, Hiroshi Qadota, Andrew D. Chisholm, Asako Sugimoto

Research output: Contribution to journalArticlepeer-review

17 Citations (Scopus)

Abstract

eyes absent/Eya is a conserved transcriptional coactivator involved in development of various tissues and organs in arthropods and vertebrates. In Drosophila eye development, eya functions as part of the transcriptional regulatory network along with eyeless/Pax6, sine oculis/Six and dachshund/Dach. Here, we present the first functional study of the C. elegans Eya homolog, EYA-1. Loss of EYA-1 function by RNAi and deletion mutations resulted in early larval lethality with incomplete penetrance, associated with defects of differentiation and morphogenesis of several tissues and organs. In late embryogenesis, morphological defect in the head region, pharyngeal malformation and excess cell deaths in the anterior region were observed. Consistently, EYA-1 was expressed in the nuclei of a subset of anterior cells including pharyngeal and body wall muscle cells, starting from the morphogenesis stage in embryogenesis. Interestingly, eya-1 and pax-6/Pax6 mutants showed a strong genetic interaction for larval viability and embryonic anterior morphogenesis. Thus, eya-1 appears to play a partially redundant role with pax-6 during C. elegans embryogenesis.

Original languageEnglish
Pages (from-to)452-463
Number of pages12
JournalDevelopmental Biology
Volume286
Issue number2
DOIs
Publication statusPublished - 2005 Oct 15

Keywords

  • Caenorhabditis elegans
  • Eya
  • Morphogenesis
  • Pax6
  • Transcriptional regulation
  • eyes absent

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology
  • Cell Biology

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