The brain-specific actin-related protein ArpNα interacts with the transcriptional co-repressor CtBP

Yukako Oma, Katsuhiko Nishimori, Masahiko Harata

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Actin-related protein (Arp) is found in many chromatin remodeling and histone acetyltransferase complexes. We previously identified ArpNα as an isoform of ArpNβ/BAF53, which is included in mammalian SWI/SNF chromatin remodeling complex, and showed that ArpNα is a potential component of the complex. Although it has a structure highly similar to ArpNβ/BAF53, ArpNα is expressed exclusively in brain and in neural differentiated embryonal carcinoma cells. Since ArpNα possesses a region that shows low similarity to ArpNβ/BAF53, we hypothesized that proteins interacting with this region contribute to the ArpNα-specific function in brain. Here we showed that ArpNα, but not ArpNβ/BAF53, interacts with the transcriptional co-repressor CtBP (C-terminal binding protein). Transactivation by the SWI/SNF complex and glucocorticoid receptor was repressed by the CtBP in the presence of ArpNα. These findings suggest that SWI/SNF complex containing ArpNα might regulate certain genes involved in brain development and/or its function differently from SWI/SNF complex containing ArpNβ/BAF53.

Original languageEnglish
Pages (from-to)521-528
Number of pages8
JournalBiochemical and biophysical research communications
Volume301
Issue number2
DOIs
Publication statusPublished - 2003 Feb 7

Keywords

  • Actin-related protein
  • Brain-specific expression
  • Chromatin
  • Chromatin remodeling
  • Co-repressor
  • CtBP
  • Protein-protein interaction

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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