The augmenting effect of OK432-stimulated B cells on the in vitro generation of anti-tumor cytotoxic T lymphocytes from tumor-draining lymph node cells: The possible role of interleukin-12

Yoshihiro Shinomiya, Mamoru Harada, Koji Tamada, Shin Kurosawa, Tadao Okamoto, Hiroshi Terao, Mitsuhiro Takenoyama, Osamu Ito, Chikashi Hirashima, Teili Li, Takayuki Shirakusa, Kikuo Nomoto

    Research output: Contribution to journalArticle

    2 Citations (Scopus)

    Abstract

    Effect of a local injection with a streptococcal preparation OK432 on the in vitro generation of anti-tumor cytotoxic T lymphocytes (CTLs) from tumor-draining lymph nodes (LN) was investigated. A peritumoral injection with OK432 on days 2, 4, 6 and 8 significantly increased both the total cell number and the proportion of B cells in the draining LN cells on day 10 after a subcutaneous inoculation with B16 melanoma. In an in vitro proliferative assay, OK432 showed a stimulatory effect on both normal splenic T and B cells. In a cytolytic assay, the OK432-injected B16-draining LN cells showed a higher level of anti-B16 CTL activity than the B16-draining LN cells after in vitro restimulation. This augmenting effect of OK432 was dependent on the B cells. Moreover, nonadherent cells from the OK432-injected B16-draining LN cells showed a low but significantly higher level of anti-B16 CTL activity than those from the B16-draining LN cells after in vitro restimulation, whereas this augmenting effect of OK432 was abolished by the in vitro addition of anti-interleukin (IL)-12 monoclonal antibody. Collectively, these findings suggest that the augmenting effect of a local injection with OK432 on the potential of tumor-draining LN cells to turn into anti-tumor CTLs after in vitro restimulation was at least in part due to IL-12 derived from the OK432-stimulated B cells.

    Original languageEnglish
    Pages (from-to)1-8
    Number of pages8
    JournalIn Vivo
    Volume11
    Issue number1
    Publication statusPublished - 1997 Jan 1

    Keywords

    • B cells
    • CTL
    • Draining LN
    • IL-12
    • OK432

    ASJC Scopus subject areas

    • Biochemistry, Genetics and Molecular Biology(all)
    • Pharmacology

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  • Cite this

    Shinomiya, Y., Harada, M., Tamada, K., Kurosawa, S., Okamoto, T., Terao, H., Takenoyama, M., Ito, O., Hirashima, C., Li, T., Shirakusa, T., & Nomoto, K. (1997). The augmenting effect of OK432-stimulated B cells on the in vitro generation of anti-tumor cytotoxic T lymphocytes from tumor-draining lymph node cells: The possible role of interleukin-12. In Vivo, 11(1), 1-8.