TY - JOUR
T1 - The arabidopsis voltage-dependent anion channel 2 is required for plant growth
AU - Tateda, Chika
AU - Kusano, Tomonobu
AU - Takahashi, Yoshihiro
N1 - Funding Information:
We thank Masahiro Yano for providing vectors and Thomas Berberich for critically reading the manuscript. This work was supported in part by Grant-in-Aid from Japan Society for the Promotion of Science to CT (21–6801).
PY - 2012
Y1 - 2012
N2 - The voltage-dependent anion channels (VDACs), known as a major group of outer mitochondrial membrane proteins, are present in all eukaryotic species. In mammalian cells, they have been established as a key player in mitochondrial metabolism and apoptosis regulation. By contrast, little is known about the function of plant VDACs. Recently, we performed functional analysis of all VDAC gene members in Arabidopsis thaliana, and revealed that each AtVDAC member has a specialized function. Especially, in spite of similar subcellular localization and expression profiling of AtVDAC2 and AtVDAC4, both the T-DNA insertion knockout mutants of them, vdac2-2 and vdac4-2, showed severe growth retardation. These results suggest that AtVDAC2 and AtVDAC4 proteins clearly have distinct functions. Here, we introduced the AtVDAC2 gene into the vdac2-2 mutant, and demonstrated that the miniature phenotype of vdac2-2 plant is abolished by AtVDAC2 expression.
AB - The voltage-dependent anion channels (VDACs), known as a major group of outer mitochondrial membrane proteins, are present in all eukaryotic species. In mammalian cells, they have been established as a key player in mitochondrial metabolism and apoptosis regulation. By contrast, little is known about the function of plant VDACs. Recently, we performed functional analysis of all VDAC gene members in Arabidopsis thaliana, and revealed that each AtVDAC member has a specialized function. Especially, in spite of similar subcellular localization and expression profiling of AtVDAC2 and AtVDAC4, both the T-DNA insertion knockout mutants of them, vdac2-2 and vdac4-2, showed severe growth retardation. These results suggest that AtVDAC2 and AtVDAC4 proteins clearly have distinct functions. Here, we introduced the AtVDAC2 gene into the vdac2-2 mutant, and demonstrated that the miniature phenotype of vdac2-2 plant is abolished by AtVDAC2 expression.
KW - Arabidopsis thaliana
KW - AtVDAC2
KW - AtVDAC4
KW - Mitochondrial porin signature
KW - Plant growth
KW - Voltage-dependent anion channel
UR - http://www.scopus.com/inward/record.url?scp=84856728863&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84856728863&partnerID=8YFLogxK
U2 - 10.4161/psb.7.1.18394
DO - 10.4161/psb.7.1.18394
M3 - Article
AN - SCOPUS:84856728863
VL - 7
SP - 31
EP - 33
JO - Plant Signaling and Behavior
JF - Plant Signaling and Behavior
SN - 1559-2316
IS - 1
ER -