The Arabidopsis TAC Position Viewer: A high-resolution map of transformation-competent artificial chromosome (TAC) clones aligned with the Arabidopsis thaliana Columbia-0 genome

Yoshitsugu Hirose, Kunihiro Suda, Yao Guang Liu, Shusei Sato, Yukino Nakamura, Koji Yokoyama, Naoki Yamamoto, Shigeru Hanano, Eiji Takita, Nozomu Sakurai, Hideyuki Suzuki, Yasukazu Nakamura, Takakazu Kaneko, Kentaro Yano, Satoshi Tabata, Daisuke Shibata

    Research output: Contribution to journalArticlepeer-review

    Abstract

    We present a high-resolution map of genomic transformation-competent artificial chromosome (TAC) clones extending over all Arabidopsis thaliana (Arabidopsis) chromosomes. The Arabidopsis genomic TAC clones have been valuable genetic tools. Previously, we constructed an Arabidopsis genomic TAC library consisting of more than 10 000 TAC clones harboring large genomic DNA fragments extending over the whole Arabidopsis genome. Here, we determined 13 577 end sequences from 6987 Arabidopsis TAC clones and mapped 5937 TAC clones to precise locations, covering approximately 90% of the Arabidopsis chromosomes. We present the large-scale data set of TAC clones with high-resolution mapping information as a Java application tool, the Arabidopsis TAC Position Viewer, which provides ready-to-go transformable genomic DNA clones corresponding to certain loci on Arabidopsis chromosomes. The TAC clone resources will accelerate genomic DNA cloning, positional walking, complementation of mutants and DNA transformation for heterologous gene expression.

    Original languageEnglish
    Pages (from-to)1114-1122
    Number of pages9
    JournalPlant Journal
    Volume83
    Issue number6
    DOIs
    Publication statusPublished - 2015 Sep 1

    Keywords

    • Arabidopsis TAC clones
    • Arabidopsis TAC library
    • Arabidopsis genome
    • Arabidopsis thaliana
    • GBrowse
    • bioinformatics
    • genomics
    • transformation-competent artificial chromosome

    ASJC Scopus subject areas

    • Genetics
    • Plant Science
    • Cell Biology

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