Abstract
Amino acid derivatives of fluorescein isothiocyanate have been separated by reverse-phase high-performance liquid chromatography in 45 min, using a linear gradient formed from acetone and 10 mm sodium phosphate buffer (pH 7.0) at 60°C. The fluorescence of the derivatives has been used for detection giving a sensitivity of less than 0.5 pmol for a single component. Applications of this method to the sequence analyses of egg white lysozyme and salmon β-melanotropin are also described.
Original language | English |
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Pages (from-to) | 446-450 |
Number of pages | 5 |
Journal | Analytical Biochemistry |
Volume | 141 |
Issue number | 2 |
DOIs | |
Publication status | Published - 1984 Sep |
Keywords
- FITC
- HPLC
- fluorescence
- protein sequencing
ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Molecular Biology
- Cell Biology