The application of fluorescein isothiocyanate and high-performance liquid chromatography for the microsequencing of proteins and peptides

Koji Muramoto; Hisao Kamiya; Hiroshi Kawauchi

doi:10.1016/0003-2697(84)90069-1

The application of fluorescein isothiocyanate and high-performance liquid chromatography for the microsequencing of proteins and peptides

Koji Muramoto, Hisao Kamiya, Hiroshi Kawauchi

Research output: Contribution to journal › Article › peer-review

69 Citations (Scopus)

Abstract

Amino acid derivatives of fluorescein isothiocyanate have been separated by reverse-phase high-performance liquid chromatography in 45 min, using a linear gradient formed from acetone and 10 mm sodium phosphate buffer (pH 7.0) at 60°C. The fluorescence of the derivatives has been used for detection giving a sensitivity of less than 0.5 pmol for a single component. Applications of this method to the sequence analyses of egg white lysozyme and salmon β-melanotropin are also described.

Original language	English
Pages (from-to)	446-450
Number of pages	5
Journal	Analytical Biochemistry
Volume	141
Issue number	2
DOIs	https://doi.org/10.1016/0003-2697(84)90069-1
Publication status	Published - 1984 Sep

Keywords

FITC
HPLC
fluorescence
protein sequencing

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

Access to Document

10.1016/0003-2697(84)90069-1

Fingerprint Dive into the research topics of 'The application of fluorescein isothiocyanate and high-performance liquid chromatography for the microsequencing of proteins and peptides'. Together they form a unique fingerprint.

Cite this

@article{0730d04dc06d42feb285340d41d618be,

title = "The application of fluorescein isothiocyanate and high-performance liquid chromatography for the microsequencing of proteins and peptides",

abstract = "Amino acid derivatives of fluorescein isothiocyanate have been separated by reverse-phase high-performance liquid chromatography in 45 min, using a linear gradient formed from acetone and 10 mm sodium phosphate buffer (pH 7.0) at 60°C. The fluorescence of the derivatives has been used for detection giving a sensitivity of less than 0.5 pmol for a single component. Applications of this method to the sequence analyses of egg white lysozyme and salmon β-melanotropin are also described.",

keywords = "FITC, HPLC, fluorescence, protein sequencing",

author = "Koji Muramoto and Hisao Kamiya and Hiroshi Kawauchi",

year = "1984",

month = sep,

doi = "10.1016/0003-2697(84)90069-1",

language = "English",

volume = "141",

pages = "446--450",

journal = "Analytical Biochemistry",

issn = "0003-2697",

publisher = "Academic Press Inc.",

number = "2",

}

TY - JOUR

T1 - The application of fluorescein isothiocyanate and high-performance liquid chromatography for the microsequencing of proteins and peptides

AU - Muramoto, Koji

AU - Kamiya, Hisao

AU - Kawauchi, Hiroshi

PY - 1984/9

Y1 - 1984/9

N2 - Amino acid derivatives of fluorescein isothiocyanate have been separated by reverse-phase high-performance liquid chromatography in 45 min, using a linear gradient formed from acetone and 10 mm sodium phosphate buffer (pH 7.0) at 60°C. The fluorescence of the derivatives has been used for detection giving a sensitivity of less than 0.5 pmol for a single component. Applications of this method to the sequence analyses of egg white lysozyme and salmon β-melanotropin are also described.

AB - Amino acid derivatives of fluorescein isothiocyanate have been separated by reverse-phase high-performance liquid chromatography in 45 min, using a linear gradient formed from acetone and 10 mm sodium phosphate buffer (pH 7.0) at 60°C. The fluorescence of the derivatives has been used for detection giving a sensitivity of less than 0.5 pmol for a single component. Applications of this method to the sequence analyses of egg white lysozyme and salmon β-melanotropin are also described.

KW - FITC

KW - HPLC

KW - fluorescence

KW - protein sequencing

UR - http://www.scopus.com/inward/record.url?scp=0021484987&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021484987&partnerID=8YFLogxK

U2 - 10.1016/0003-2697(84)90069-1

DO - 10.1016/0003-2697(84)90069-1

M3 - Article

C2 - 6437275

AN - SCOPUS:0021484987

VL - 141

SP - 446

EP - 450

JO - Analytical Biochemistry

JF - Analytical Biochemistry

SN - 0003-2697

IS - 2

ER -

The application of fluorescein isothiocyanate and high-performance liquid chromatography for the microsequencing of proteins and peptides

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Cite this