The application of fluorescein isothiocyanate and high-performance liquid chromatography for the microsequencing of proteins and peptides

Koji Muramoto; Hisao Kamiya; Hiroshi Kawauchi

doi:10.1016/0003-2697(84)90069-1

The application of fluorescein isothiocyanate and high-performance liquid chromatography for the microsequencing of proteins and peptides

Koji Muramoto, Hisao Kamiya, Hiroshi Kawauchi

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69 Citations (Scopus)

Abstract

Amino acid derivatives of fluorescein isothiocyanate have been separated by reverse-phase high-performance liquid chromatography in 45 min, using a linear gradient formed from acetone and 10 mm sodium phosphate buffer (pH 7.0) at 60°C. The fluorescence of the derivatives has been used for detection giving a sensitivity of less than 0.5 pmol for a single component. Applications of this method to the sequence analyses of egg white lysozyme and salmon β-melanotropin are also described.

Original language	English
Pages (from-to)	446-450
Number of pages	5
Journal	Analytical Biochemistry
Volume	141
Issue number	2
DOIs	https://doi.org/10.1016/0003-2697(84)90069-1
Publication status	Published - 1984 Sep

Keywords

FITC
HPLC
fluorescence
protein sequencing

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/0003-2697(84)90069-1

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author = "Koji Muramoto and Hisao Kamiya and Hiroshi Kawauchi",

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AU - Muramoto, Koji

AU - Kamiya, Hisao

AU - Kawauchi, Hiroshi

PY - 1984/9

Y1 - 1984/9

N2 - Amino acid derivatives of fluorescein isothiocyanate have been separated by reverse-phase high-performance liquid chromatography in 45 min, using a linear gradient formed from acetone and 10 mm sodium phosphate buffer (pH 7.0) at 60°C. The fluorescence of the derivatives has been used for detection giving a sensitivity of less than 0.5 pmol for a single component. Applications of this method to the sequence analyses of egg white lysozyme and salmon β-melanotropin are also described.

AB - Amino acid derivatives of fluorescein isothiocyanate have been separated by reverse-phase high-performance liquid chromatography in 45 min, using a linear gradient formed from acetone and 10 mm sodium phosphate buffer (pH 7.0) at 60°C. The fluorescence of the derivatives has been used for detection giving a sensitivity of less than 0.5 pmol for a single component. Applications of this method to the sequence analyses of egg white lysozyme and salmon β-melanotropin are also described.

KW - FITC

KW - HPLC

KW - fluorescence

KW - protein sequencing

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ER -

The application of fluorescein isothiocyanate and high-performance liquid chromatography for the microsequencing of proteins and peptides

Abstract

Keywords

ASJC Scopus subject areas

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