The application of fluorescein isothiocyanate and high-performance liquid chromatography for the microsequencing of proteins and peptides

Koji Muramoto, Hisao Kamiya, Hiroshi Kawauchi

    Research output: Contribution to journalArticlepeer-review

    69 Citations (Scopus)

    Abstract

    Amino acid derivatives of fluorescein isothiocyanate have been separated by reverse-phase high-performance liquid chromatography in 45 min, using a linear gradient formed from acetone and 10 mm sodium phosphate buffer (pH 7.0) at 60°C. The fluorescence of the derivatives has been used for detection giving a sensitivity of less than 0.5 pmol for a single component. Applications of this method to the sequence analyses of egg white lysozyme and salmon β-melanotropin are also described.

    Original languageEnglish
    Pages (from-to)446-450
    Number of pages5
    JournalAnalytical Biochemistry
    Volume141
    Issue number2
    DOIs
    Publication statusPublished - 1984 Sep

    Keywords

    • FITC
    • HPLC
    • fluorescence
    • protein sequencing

    ASJC Scopus subject areas

    • Biophysics
    • Biochemistry
    • Molecular Biology
    • Cell Biology

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