TY - JOUR
T1 - The antitumor activity induced by the in vivo administration of activated B cells bound to anti-CD3 monoclonal antibody
AU - Harada, Mamoru
AU - Okamoto, Tadao
AU - Kurosawa, Shin
AU - Shinomiya, Yoshihiro
AU - Ito, Osamu
AU - Takenoyama, Mitsuhiro
AU - Terao, Hiroshi
AU - Matsuzaki, Goro
AU - Kimura, Genki
AU - Nomoto, Kikuo
PY - 1995/3
Y1 - 1995/3
N2 - We examined the immunotherapeutic ability of activated B cells which hound to anti-CD3 monoclonal antibody (mAb) to enhance antitumor T cell immunity in vivo. A flow cytometric analysis revealed that LPS (lipopolysaccharide)-activated B cells (LPS blasts) expressed Fc receptor (FcR) which can bind to anti-CD3 mAb, LPS blasts were also stained with CTLA-4Ig, which can bind to costimulation molecules with high affinity, which suggested that LPS blasts expressed costimulation molecules on their surface. In an in vitro. assay, T cells remarkably proliferated in the presence of LPS blasts and soluble anti-CD3 mAb, whereas this proliferation was blocked by the addition of CTLA-4Ig. In a model of metastasis established by the intravenous inoculation of melanoma cells, the in vivo administration of LPS blasts incubated with anti-CD3 mAb and followed by treatment with polyethylene glycol, to reinforce the binding, induced a low but significant antitumor activity against melanoma. The antitumor activity induced by the in vivo administration of LPS blasts which bound to anti-CD3 mAb was also detected in the spontaneously established model of metastasis. These results therefore suggest that the in vivo administration of activated B cells which bound to anti-CD3 mAb was able to enhance the antitumor T cell response against metastatic melanoma.
AB - We examined the immunotherapeutic ability of activated B cells which hound to anti-CD3 monoclonal antibody (mAb) to enhance antitumor T cell immunity in vivo. A flow cytometric analysis revealed that LPS (lipopolysaccharide)-activated B cells (LPS blasts) expressed Fc receptor (FcR) which can bind to anti-CD3 mAb, LPS blasts were also stained with CTLA-4Ig, which can bind to costimulation molecules with high affinity, which suggested that LPS blasts expressed costimulation molecules on their surface. In an in vitro. assay, T cells remarkably proliferated in the presence of LPS blasts and soluble anti-CD3 mAb, whereas this proliferation was blocked by the addition of CTLA-4Ig. In a model of metastasis established by the intravenous inoculation of melanoma cells, the in vivo administration of LPS blasts incubated with anti-CD3 mAb and followed by treatment with polyethylene glycol, to reinforce the binding, induced a low but significant antitumor activity against melanoma. The antitumor activity induced by the in vivo administration of LPS blasts which bound to anti-CD3 mAb was also detected in the spontaneously established model of metastasis. These results therefore suggest that the in vivo administration of activated B cells which bound to anti-CD3 mAb was able to enhance the antitumor T cell response against metastatic melanoma.
UR - http://www.scopus.com/inward/record.url?scp=0028948414&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0028948414&partnerID=8YFLogxK
U2 - 10.1006/cimm.1995.1017
DO - 10.1006/cimm.1995.1017
M3 - Article
C2 - 7867078
AN - SCOPUS:0028948414
VL - 161
SP - 132
EP - 137
JO - Cellular Immunology
JF - Cellular Immunology
SN - 0008-8749
IS - 1
ER -