The antitumor activity induced by the in vivo administration of activated B cells bound to anti-CD3 monoclonal antibody

Mamoru Harada, Tadao Okamoto, Shin Kurosawa, Yoshihiro Shinomiya, Osamu Ito, Mitsuhiro Takenoyama, Hiroshi Terao, Goro Matsuzaki, Genki Kimura, Kikuo Nomoto

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    13 Citations (Scopus)

    Abstract

    We examined the immunotherapeutic ability of activated B cells which hound to anti-CD3 monoclonal antibody (mAb) to enhance antitumor T cell immunity in vivo. A flow cytometric analysis revealed that LPS (lipopolysaccharide)-activated B cells (LPS blasts) expressed Fc receptor (FcR) which can bind to anti-CD3 mAb, LPS blasts were also stained with CTLA-4Ig, which can bind to costimulation molecules with high affinity, which suggested that LPS blasts expressed costimulation molecules on their surface. In an in vitro. assay, T cells remarkably proliferated in the presence of LPS blasts and soluble anti-CD3 mAb, whereas this proliferation was blocked by the addition of CTLA-4Ig. In a model of metastasis established by the intravenous inoculation of melanoma cells, the in vivo administration of LPS blasts incubated with anti-CD3 mAb and followed by treatment with polyethylene glycol, to reinforce the binding, induced a low but significant antitumor activity against melanoma. The antitumor activity induced by the in vivo administration of LPS blasts which bound to anti-CD3 mAb was also detected in the spontaneously established model of metastasis. These results therefore suggest that the in vivo administration of activated B cells which bound to anti-CD3 mAb was able to enhance the antitumor T cell response against metastatic melanoma.

    Original languageEnglish
    Pages (from-to)132-137
    Number of pages6
    JournalCellular Immunology
    Volume161
    Issue number1
    DOIs
    Publication statusPublished - 1995 Mar

    ASJC Scopus subject areas

    • Immunology

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  • Cite this

    Harada, M., Okamoto, T., Kurosawa, S., Shinomiya, Y., Ito, O., Takenoyama, M., Terao, H., Matsuzaki, G., Kimura, G., & Nomoto, K. (1995). The antitumor activity induced by the in vivo administration of activated B cells bound to anti-CD3 monoclonal antibody. Cellular Immunology, 161(1), 132-137. https://doi.org/10.1006/cimm.1995.1017