The ALS/FTLD-related RNA-binding proteins TDP-43 and FUS have common downstream RNA targets in cortical neurons

Daiyu Honda; Shinsuke Ishigaki; Yohei Iguchi; Yusuke Fujioka; Tsuyoshi Udagawa; Akio Masuda; Kinji Ohno; Masahisa Katsuno; Gen Sobue

doi:10.1016/j.fob.2013.11.001

The ALS/FTLD-related RNA-binding proteins TDP-43 and FUS have common downstream RNA targets in cortical neurons

Daiyu Honda, Shinsuke Ishigaki, Yohei Iguchi, Yusuke Fujioka, Tsuyoshi Udagawa, Akio Masuda, Kinji Ohno, Masahisa Katsuno, Gen Sobue

Research output: Contribution to journal › Article › peer-review

42 Citations (Scopus)

Abstract

TDP-43 and FUS are linked to amyotrophic lateral sclerosis (ALS) and frontotemporal lobar degeneration (FTLD), and loss of function of either protein contributes to these neurodegenerative conditions. To elucidate the TDP-43- and FUS-regulated pathophysiological RNA metabolism cascades, we assessed the differential gene expression and alternative splicing profiles related to regulation by either TDP-43 or FUS in primary cortical neurons. These profiles overlapped by >25% with respect to gene expression and >9% with respect to alternative splicing. The shared downstream RNA targets of TDP-43 and FUS may form a common pathway in the neurodegenerative processes of ALS/FTLD.

Original language	English
Pages (from-to)	1-10
Number of pages	10
Journal	FEBS Open Bio
Volume	4
DOIs	https://doi.org/10.1016/j.fob.2013.11.001
Publication status	Published - 2014
Externally published	Yes

Keywords

ALS
FTLD
FUS
TDP-43
Transcriptome

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)

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10.1016/j.fob.2013.11.001

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title = "The ALS/FTLD-related RNA-binding proteins TDP-43 and FUS have common downstream RNA targets in cortical neurons",

abstract = "TDP-43 and FUS are linked to amyotrophic lateral sclerosis (ALS) and frontotemporal lobar degeneration (FTLD), and loss of function of either protein contributes to these neurodegenerative conditions. To elucidate the TDP-43- and FUS-regulated pathophysiological RNA metabolism cascades, we assessed the differential gene expression and alternative splicing profiles related to regulation by either TDP-43 or FUS in primary cortical neurons. These profiles overlapped by >25% with respect to gene expression and >9% with respect to alternative splicing. The shared downstream RNA targets of TDP-43 and FUS may form a common pathway in the neurodegenerative processes of ALS/FTLD.",

keywords = "ALS, FTLD, FUS, TDP-43, Transcriptome",

author = "Daiyu Honda and Shinsuke Ishigaki and Yohei Iguchi and Yusuke Fujioka and Tsuyoshi Udagawa and Akio Masuda and Kinji Ohno and Masahisa Katsuno and Gen Sobue",

note = "Funding Information: Part of this study represents the results of the “Integrated Research on Neuropsychiatric Disorders” project, which has been conducted under the Strategic Research Program for Brain Sciences of the Ministry of Education, Culture, Sports, Science and Technology of Japan. This work was also supported by grants-in-aid from the CREST/JST , MEXT , and MHLW of Japan . ",

year = "2014",

doi = "10.1016/j.fob.2013.11.001",

language = "English",

volume = "4",

pages = "1--10",

journal = "FEBS Open Bio",

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T1 - The ALS/FTLD-related RNA-binding proteins TDP-43 and FUS have common downstream RNA targets in cortical neurons

AU - Honda, Daiyu

AU - Ishigaki, Shinsuke

AU - Iguchi, Yohei

AU - Fujioka, Yusuke

AU - Udagawa, Tsuyoshi

AU - Masuda, Akio

AU - Ohno, Kinji

AU - Katsuno, Masahisa

AU - Sobue, Gen

N1 - Funding Information: Part of this study represents the results of the “Integrated Research on Neuropsychiatric Disorders” project, which has been conducted under the Strategic Research Program for Brain Sciences of the Ministry of Education, Culture, Sports, Science and Technology of Japan. This work was also supported by grants-in-aid from the CREST/JST , MEXT , and MHLW of Japan .

PY - 2014

Y1 - 2014

N2 - TDP-43 and FUS are linked to amyotrophic lateral sclerosis (ALS) and frontotemporal lobar degeneration (FTLD), and loss of function of either protein contributes to these neurodegenerative conditions. To elucidate the TDP-43- and FUS-regulated pathophysiological RNA metabolism cascades, we assessed the differential gene expression and alternative splicing profiles related to regulation by either TDP-43 or FUS in primary cortical neurons. These profiles overlapped by >25% with respect to gene expression and >9% with respect to alternative splicing. The shared downstream RNA targets of TDP-43 and FUS may form a common pathway in the neurodegenerative processes of ALS/FTLD.

AB - TDP-43 and FUS are linked to amyotrophic lateral sclerosis (ALS) and frontotemporal lobar degeneration (FTLD), and loss of function of either protein contributes to these neurodegenerative conditions. To elucidate the TDP-43- and FUS-regulated pathophysiological RNA metabolism cascades, we assessed the differential gene expression and alternative splicing profiles related to regulation by either TDP-43 or FUS in primary cortical neurons. These profiles overlapped by >25% with respect to gene expression and >9% with respect to alternative splicing. The shared downstream RNA targets of TDP-43 and FUS may form a common pathway in the neurodegenerative processes of ALS/FTLD.

KW - ALS

KW - FTLD

KW - FUS

KW - TDP-43

KW - Transcriptome

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DO - 10.1016/j.fob.2013.11.001

M3 - Article

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VL - 4

SP - 1

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JO - FEBS Open Bio

JF - FEBS Open Bio

ER -

The ALS/FTLD-related RNA-binding proteins TDP-43 and FUS have common downstream RNA targets in cortical neurons

Abstract

Keywords

ASJC Scopus subject areas

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