The advantages of cDNA microarray as an effective tool for identification of reproductive organ-specific genes in a model legume, Lotus japonicus

Makoto Endo, Hitoshi Matsubara, Takao Kokubun, Hiromi Masuko, Yoshihito Takahata, Tohru Tsuchiya, Hiroo Fukuda, Taku Demura, Masao Watanabe

Research output: Contribution to journalArticlepeer-review

53 Citations (Scopus)

Abstract

To understand the molecular mechanisms intrinsic to reproductive organ development a cDNA microarray, fabricated from flower bud cDNA clones, was used to isolate genes, which are specifically expressed during the development of the anther and pistil in Lotus japonicus. Cluster analysis of the microarray data revealed 21 and 111 independent cDNA groups, which were specifically expressed in immature and mature anthers, respectively. RT-PCR was performed to provide a direct assessment of the accuracy and reproducibility of our approach. Confirmation of our results suggests that cDNA microarray technology is an effective tool for identification of novel reproductive organ-specific genes.

Original languageEnglish
Pages (from-to)229-237
Number of pages9
JournalFEBS Letters
Volume514
Issue number2-3
DOIs
Publication statusPublished - 2002 Mar 13
Externally publishedYes

Keywords

  • Anther-specific gene
  • Lotus japonicus
  • Pistil-specific gene
  • Reverse transcriptase polymerase chain reaction
  • cDNA microarray

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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