The ADP/ATP ratio: A novel predictive assay for quality assessment of isolated pancreatic islets

M. Goto, J. Holgersson, M. Kumagai-Braesch, O. Korsgren

Research output: Contribution to journalArticlepeer-review

81 Citations (Scopus)

Abstract

The current standard assays for islet product release criteria are unable to predict the outcome after clinical islet transplantation. Therefore, establishment of reliable and rapid assays enabling pre-transplantation prediction of islet potency is warranted. In the present study, we have evaluated the adenosine diphosphate (ADP)/adenosine triphosphate (ATP) test, the glucose-stimulated insulin release, the loss of islets during the first 24 h in culture, and the insulin/deoxyribonucleic acid as predictive assays for the ability of isolated porcine islets to cure athymic mice with streptozotocin-induced diabetes. From the results presented, it is concluded that the measurement of the ADP/ATP ratio was the only test that correlated with transplantation outcome. In summary, we propose that the ADP/ATP assay is worthwhile as applied to human islet transplantation and seek to validate it as a rapid and potent predictor of transplant outcome.

Original languageEnglish
Pages (from-to)2483-2487
Number of pages5
JournalAmerican Journal of Transplantation
Volume6
Issue number10
DOIs
Publication statusPublished - 2006 Oct

Keywords

  • ADP/ATP ratio
  • Adult porcine islets
  • Islet transplantation
  • Viability

ASJC Scopus subject areas

  • Immunology and Allergy
  • Transplantation
  • Pharmacology (medical)

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