TY - JOUR
T1 - The actin family protein ARP6 contributes to the structure and the function of the nucleolus
AU - Kitamura, Hiroshi
AU - Matsumori, Haruka
AU - Kalendova, Alzbeta
AU - Hozak, Pavel
AU - Goldberg, Ilya G.
AU - Nakao, Mitsuyoshi
AU - Saitoh, Noriko
AU - Harata, Masahiko
N1 - Funding Information:
We thank all the members of the laboratory for the discussions. This work was supported by Grants-in-Aid for Scientific Research on Innovative Areas ( 25116009 ) (M.H. and N.S.) and the Human Frontier Science Program ( RGP0017 ) (M.H.). H. K. and H. M. thank the Japan Society for the Promotion of Science (JSPS) for Young Scientist Fellowships.
Funding Information:
P. H. was supported by Human Frontier in Science programe ( RGP0017/2013 ), A. K. by Ministry of Education, Youth and Sports of the Czech Republic ( CZ.1.07/2.3.00/30.0050 ). This publication is supported by the project “ BIOCEV – Biotechnology and Biomedicine Centre of the Academy of Sciences and Charles University ” ( CZ.1.05/1.1.00/02.0109 ), from the European Regional Development Fund and with institutional support ( RVO68378050 ).
Funding Information:
IGG is supported by the Intramural Research Program of the U.S. National Institutes of Health, National Institute on Aging.
PY - 2015/7/30
Y1 - 2015/7/30
N2 - The actin family members, consisting of actin and actin-related proteins (ARPs), are essential components of chromatin remodeling complexes. ARP6, one of the nuclear ARPs, is part of the Snf-2-related CREB-binding protein activator protein (SRCAP) chromatin remodeling complex, which promotes the deposition of the histone variant H2A.Z into the chromatin. In this study, we showed that ARP6 influences the structure and the function of the nucleolus. ARP6 is localized in the central region of the nucleolus, and its knockdown induced a morphological change in the nucleolus. We also found that in the presence of high concentrations of glucose ARP6 contributed to the maintenance of active ribosomal DNA (rDNA) transcription by placing H2A.Z into the chromatin. In contrast, under starvation, ARP6 was required for cell survival through the repression of rDNA transcription independently of H2A.Z. These findings reveal novel pleiotropic roles for the actin family in nuclear organization and metabolic homeostasis.
AB - The actin family members, consisting of actin and actin-related proteins (ARPs), are essential components of chromatin remodeling complexes. ARP6, one of the nuclear ARPs, is part of the Snf-2-related CREB-binding protein activator protein (SRCAP) chromatin remodeling complex, which promotes the deposition of the histone variant H2A.Z into the chromatin. In this study, we showed that ARP6 influences the structure and the function of the nucleolus. ARP6 is localized in the central region of the nucleolus, and its knockdown induced a morphological change in the nucleolus. We also found that in the presence of high concentrations of glucose ARP6 contributed to the maintenance of active ribosomal DNA (rDNA) transcription by placing H2A.Z into the chromatin. In contrast, under starvation, ARP6 was required for cell survival through the repression of rDNA transcription independently of H2A.Z. These findings reveal novel pleiotropic roles for the actin family in nuclear organization and metabolic homeostasis.
KW - ARP6
KW - Actin-related protein
KW - Histone H2A.Z
KW - Nucleolus
KW - Wndchrm
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U2 - 10.1016/j.bbrc.2015.07.005
DO - 10.1016/j.bbrc.2015.07.005
M3 - Article
C2 - 26164235
AN - SCOPUS:84938383672
VL - 464
SP - 554
EP - 560
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 2
ER -