The accessory subunit of human DNA polymerase γ is required for mitochondrial DNA maintenance and is able to stabilize the catalytic subunit

Yura Do, Shigeru Matsuda, Teppei Inatomi, Kazuto Nakada, Takehiro Yasukawa, Dongchon Kang

Research output: Contribution to journalArticlepeer-review

Abstract

Human DNA polymerase γ (POLG) is a mitochondria-specific replicative DNA polymerase consisting of a single catalytic subunit, POLGα, and a dimeric accessory subunit, POLGβ. To gain a deeper understanding of the role of POLGβ, we knocked out this protein in cultured human cybrid cells and established numerous knockout clones. POLGβ-knockout clones presented a clear phenotype of mitochondrial DNA loss, indicating that POLGβ is necessary for mitochondrial DNA replication. Moreover, POLGβ-knockout cells showed a severe decrease in POLGα levels and acute suppression of POLGβ expression efficiently down-regulated POLGα levels. These results suggest that, in addition to its role as the processivity factor of POLG, POLGβ acts as a POLGα stabilizer, an important role for POLGβ in mitochondrial DNA maintenance.

Original languageEnglish
Pages (from-to)133-139
Number of pages7
JournalMitochondrion
Volume53
DOIs
Publication statusPublished - 2020 Jul
Externally publishedYes

Keywords

  • Cybrid cells
  • DNA polymerase γ
  • Mitochondrial DNA
  • MtDNA replication
  • POLGβ

ASJC Scopus subject areas

  • Molecular Medicine
  • Molecular Biology
  • Cell Biology

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